Isocratic, simultaneous normal-phase LC separation of four impurities for a stavudine synthesis control

The isocratic separation of impurities for a stavudine synthesis control has been studied by liquid chromatography (LC). The investigations have shown that isocratic reversed-phase LC conditions were unable to achieve a good separation. It was established that isocratic normal-phase LC system with common silica column as a stationary phase and the mixture of ethyl acetate with 4% (v/v) of ethylene glycol as a mobile phase is useful in simultaneous determination of four impurities for a stavudine synthesis control.