کد مقاله | کد نشریه | سال انتشار | مقاله انگلیسی | نسخه تمام متن |
---|---|---|---|---|
10564378 | 970687 | 2005 | 10 صفحه PDF | دانلود رایگان |
عنوان انگلیسی مقاله ISI
LC-tandem MS in proteome characterization
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کلمات کلیدی
موضوعات مرتبط
مهندسی و علوم پایه
شیمی
شیمی آنالیزی یا شیمی تجزیه
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چکیده انگلیسی
The coupling of high-performance liquid chromatography (HPLC) to mass spectrometry (MS) has been routinely used for a number of years for the analysis of a wide variety of different compounds. In typical proteomic analyses, where enzymatic digestion is used to generate proteolytic peptides, the limited amount of sample restricts the utility of conventional HPLC methods for MS detection. As reduced column diameters and nanolitre per minute flow rates have become increasingly standard, the application of HPLC to the analysis of low-volume, low-abundance samples has now become readily achievable. A number of novel chromatographic methods have increased the utility of such approaches for proteome-wide analyses. However, there remain in proteomic analyses some important challenges, which are being addressed by state-of-the-art methodologies. This article reviews a number of pertinent considerations and technological advances in proteomic analyses using HPLC-tandem MS (MS2).
ناشر
Database: Elsevier - ScienceDirect (ساینس دایرکت)
Journal: TrAC Trends in Analytical Chemistry - Volume 24, Issue 7, JulyâAugust 2005, Pages 566-575
Journal: TrAC Trends in Analytical Chemistry - Volume 24, Issue 7, JulyâAugust 2005, Pages 566-575
نویسندگان
Sarah R. Hart, Simon J. Gaskell,