کد مقاله | کد نشریه | سال انتشار | مقاله انگلیسی | نسخه تمام متن |
---|---|---|---|---|
10585810 | 981379 | 2011 | 5 صفحه PDF | دانلود رایگان |
عنوان انگلیسی مقاله ISI
'Click peptide' using production of monomer Aβ from the O-acyl isopeptide: Application to assay system of aggregation inhibitors and cellular cytotoxicity
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کلمات کلیدی
NDGAIsopeptideHFIPTFADMEM1,1,1,3,3,3-hexafluoro-2-propanol - 1،1،1،3،3،3-هگزافلوئورو 2-پروپانولDMSO - DMSOAlzheimer - آلزایمرAmyloid - آمیلوئیدTrifluoroacetic acid - اسید TrifluoroaceticTannic acid - اسید تانیکnordihydroguaiaretic acid - اسید نوردی هیدروگالیارتیInhibitor - بازدارندهThT - بلهAggregation - تجمعThioflavin-T - تیوفلاین-TDimethyl sulfoxide - دیمتیل سولفواکسیدRifampicin - ریفامپینDulbecco’s modified eagle’s medium - محیط عقاب اصلاح شده DulbeccoMyr - میرMyricetin - میریستینRif - کد عکس
موضوعات مرتبط
مهندسی و علوم پایه
شیمی
شیمی آلی
پیش نمایش صفحه اول مقاله
![عکس صفحه اول مقاله: 'Click peptide' using production of monomer Aβ from the O-acyl isopeptide: Application to assay system of aggregation inhibitors and cellular cytotoxicity 'Click peptide' using production of monomer Aβ from the O-acyl isopeptide: Application to assay system of aggregation inhibitors and cellular cytotoxicity](/preview/png/10585810.png)
چکیده انگلیسی
The O-acyl isopeptide of Aβ1-42 (1), possessing an ester bond at the Gly25-Ser26 sequence, is a water-soluble and non-aggregative precursor molecule and is capable of production of monomer Aβ1-42. The SDS-PAGE result showed that the Aβ1-42, produced from 1, adopted monomeric state at first and then self-assembled to oligomer. The oligomeric state was stabilized by nordihydroguaiaretic acid. The Thioflavin-T (ThT) fluorescence intensity derived from Aβ1-42 (generated from 1) was suppressed by various aggregation inhibitors. Finally, 1 could generate Aβ1-42 via the O-to-N acyl migration under cellular medium conditions and the produced Aβ1-42 exhibited cytotoxicity against PC12 cells. These results suggest that the click peptide system, which enables us to predominantly produce monomer Aβ1-42 under physiological conditions, would be adoptable to various biochemical and biophysical experiments including cellular system to investigate the functions of Aβ1-42.
ناشر
Database: Elsevier - ScienceDirect (ساینس دایرکت)
Journal: Bioorganic & Medicinal Chemistry - Volume 19, Issue 5, 1 March 2011, Pages 1729-1733
Journal: Bioorganic & Medicinal Chemistry - Volume 19, Issue 5, 1 March 2011, Pages 1729-1733
نویسندگان
Youhei Sohma, Yuta Hirayama, Atsuhiko Taniguchi, Hidehito Mukai, Yoshiaki Kiso,