کد مقاله | کد نشریه | سال انتشار | مقاله انگلیسی | نسخه تمام متن |
---|---|---|---|---|
10593534 | 981811 | 2013 | 4 صفحه PDF | دانلود رایگان |
عنوان انگلیسی مقاله ISI
The inhibitory effect of a synthetic compound, (Z)-5-(2,4-dihydroxybenzylidene) thiazolidine-2,4-dione (MHY498), on nitric oxide-induced melanogenesis
دانلود مقاله + سفارش ترجمه
دانلود مقاله ISI انگلیسی
رایگان برای ایرانیان
کلمات کلیدی
pKaACTHguanylate cyclaseMITFPKGpCREBα-MSHET-1cGMPUVAEtOHCREBSDScAMP - cAMPl-3,4-dihydroxyphenylalanine - L-3،4-دی هیدروکسی فنیل آلانینl-DOPA - L-DOPAl-NAME - L-NAMEadenosine 3′,5′-cyclic monophosphate - آدنوزین 3 '، 5'-سیکلیک منوفسفرهEthanol - اتانولUltraviolet - اشعه فرابنفشUltraviolet A - اشعه ماوراء بنفش Aendothelin-1 - اندوتلین-1analysis of variance - تحلیل واریانسANOVA - تحلیل واریانس Analysis of varianceTyrosinase - تیروزیناز sodium dodecyl sulphate - سدیم دودسیل سولفاتMicrophthalmia-associated transcription factor - فاکتور رونویسی مرتبط با میکرو فتالمیMelanogenesis - ملانوژنزmelanin - ملانینsodium nitroprusside - نیتروپروساید سدیمNitric oxide - نیتریک اکسیدadrenocorticotropic hormone - هورمون adrenocorticotropicalpha-melanocyte stimulating hormone - هورمون تحریک کننده آلفا ملانوستیprotein kinase A - پروتئین کیناز Aprotein kinase G - پروتئین کیناز GSNP - چندریختی تک-نوکلئوتید
موضوعات مرتبط
مهندسی و علوم پایه
شیمی
شیمی آلی
پیش نمایش صفحه اول مقاله
چکیده انگلیسی
Nitric oxide (NO) and the NO/PKG signaling pathway play crucial roles in ultraviolet (UV)-induced melanogenesis, which is known to be related to the induction of tyrosinase. In an attempt to find a novel anti-melanogenic agent, we synthesized (Z)-5-(2,4-dihydroxybenzylidene)thiazolidine-2,4-dione (MHY498). The purpose of this study was to investigate the effect of MHY498 on NO levels and on the NO-mediated signaling pathway using an in vitro model of melanogenesis. MHY498 inhibited 200 μM sodium nitroprusside (SNP, a NO donor)-induced NO generation, dose-dependently and suppressed tyrosinase activity and melanin synthesis induced by SNP in B16F10 melanoma cells. To investigate the effect of MHY498 on NO-mediated signaling pathway, guanosine cyclic 3â²,5â²-monophosphate (cGMP) activities were measured using a cGMP EIA Kit and western blotting was performed to determine the effects of MHY498 on the gene expressions of tyrosinase and microphthalmia-associated transcription factor (MITF). The increased activity of cGMP by SNP was reduced dose-dependently by pretreatment with MHY498. Furthermore, MHY498 suppressed the expressions of tyrosinase and MITF stimulated by SNP. This study shows that enhancement of tyrosinase gene expression via the cGMP pathway is a probable primary mechanism of NO-induced melanogenesis and that the NO-mediated signaling pathway with the expression of MITF enhances melanogenesis. In addition, MHY498 was found to scavenge NO and to suppress the activity of the NO-mediated signaling pathway, and thus, to subsequently down-regulate tyrosinase expression and melanogenesis. This study suggests that MHY498 is a promising anti-melanogenic agent that targets the NO-induced cGMP signaling pathway.
ناشر
Database: Elsevier - ScienceDirect (ساینس دایرکت)
Journal: Bioorganic & Medicinal Chemistry Letters - Volume 23, Issue 15, 1 August 2013, Pages 4332-4335
Journal: Bioorganic & Medicinal Chemistry Letters - Volume 23, Issue 15, 1 August 2013, Pages 4332-4335
نویسندگان
So Hee Kim, Yeon Ja Choi, Kyoung Mi Moon, Hye Jin Lee, Youngwoo Woo, Ki Wung Chung, Yuri Jung, Sora Kim, Pusoon Chun, Youngjoo Byun, Young Mi Ha, Hyung Ryong Moon, Hae Young Chung,