کد مقاله | کد نشریه | سال انتشار | مقاله انگلیسی | نسخه تمام متن |
---|---|---|---|---|
10597131 | 981955 | 2013 | 6 صفحه PDF | دانلود رایگان |
عنوان انگلیسی مقاله ISI
Ultrasonic-assisted preparation of a low molecular weight heparin (LMWH) with anticoagulant activity
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کلمات کلیدی
DepolymerizationAnti-IIaGAGsUFHLMWHSECHIT - اصابتUltrasonic waves - امواج التراسونیکHeparin-induced thrombocytopenia - ترومبوسیتوپنی ناشی از هپارینdegree of polymerization - درجه پلیمریزاسیونpolydispersity index - شاخص پلییدریزاییAnti-Xa - ضد XaAverage molecular weight - میانگین وزن مولکولیHeparin - هپارین Low molecular weight heparin - هپارین با وزن مولکولی کم unfractionated heparin - هپارین غیر اشباع شدهSize exclusion chromatography - کروماتوگرافی اندازهای طردیGlycosaminoglycans - گلیکوز آمینو گلیکان ها
موضوعات مرتبط
مهندسی و علوم پایه
شیمی
شیمی آلی
پیش نمایش صفحه اول مقاله
چکیده انگلیسی
Low molecular weight heparin (LMWH) is currently used as an anticoagulant agent and constitutes an alternative to unfractionated heparin, which is the cause of serious adverse drug reaction such as heparin-induced thrombocytopenia (HIT). Commercially available LMWH is produced by enzymatic depolymerization that is costly or by chemical methods that are generally carried out under conditions that could imply side reactions that reduce final product efficiency and yields. In this work, we present the use of a physicochemical method for the production of LMWH. This method consists in the use of hydrogen peroxide-catalyzed radical hydrolysis assisted by ultrasonic waves. LMWH that are produced using this physicochemical method have an average molecular weight and anticoagulant properties (Anti-Xa and Anti-IIa) that are comparable to some of commercial LMWH that are currently used. Ultrasonic-assisted radical depolymerization of heparin leads to products with a remarkably low polydispersity index. Moreover, in comparison to other LMWH such as those produced by enzymatic β-elimination, this physicochemical depolymerization of heparin induces fewer oligosaccharides with less than five monosaccharide units. This contributes to the better preservation of the ATIII pentasaccharide binding sequence, which results in a high Anti-Xa/Anti-IIa ratio (1.86). However, LMWH obtained using this physicochemical method have a lower degree of sulfation than other LMWH, which seems to be the cause of a lower Anti-Xa and Anti-IIa activity (143.62 ± 5.42 and 77.07 ± 4.4, respectively).
ناشر
Database: Elsevier - ScienceDirect (ساینس دایرکت)
Journal: Carbohydrate Polymers - Volume 97, Issue 2, 12 September 2013, Pages 684-689
Journal: Carbohydrate Polymers - Volume 97, Issue 2, 12 September 2013, Pages 684-689
نویسندگان
Oussama Achour, Nicolas Bridiau, Azza Godhbani, Florian Le Joubioux, Stephanie Bordenave Juchereau, Fredéric Sannier, Jean-Marie Piot, Ingrid Fruitier Arnaudin, Thierry Maugard,