Purification and characterization of chitin deacetylase from Penicillium oxalicum SAEM-51

► Chitin deacetylase with notably higher extracellular activity was purified from Penicillium oxalicum. ► Thermodynamics for chitin deacetylase denaturation was enumerated for the first time which denoted that enzyme preparation was quite stable at higher temperatures. ► Secondary structure for the first time for the enzyme was determined by far-UV CD spectroscopic analysis. ► Purified enzyme preparation was detected to be markedly active in deacetylating chitin and N-acetylglucosamine oligomers.