Demonstration of the presence of acetylation and arabinose branching as structural features of locust bean gum galactomannans

- LBG galactomannans were submitted to a mannanase hydrolysis and the resultant oligosaccharides were analysed by ESI-MS/MS.
- Acetylated hexoses and/or substituted with a pentose were found.
- These structures were confirmed by GC-qMS of reduced and permethylated oligosaccharides.
- Glycosidic-linkage analysis shows the occurrence of Araf linked at the O-6 of (1 → 4)-Manp residues.