کد مقاله | کد نشریه | سال انتشار | مقاله انگلیسی | نسخه تمام متن |
---|---|---|---|---|
10604630 | 982530 | 2011 | 9 صفحه PDF | دانلود رایگان |
عنوان انگلیسی مقاله ISI
Effect of oat and barley β-glucans on inhibition of cytokine-induced adhesion molecule expression in human aortic endothelial cells: Molecular structure-function relations
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کلمات کلیدی
EGMOBCIL-1βHBSICAM-1HPSECVCAM-1HEPESEBMintracellular cell adhesion moleculeFBShEGFDMEMPBSHPAEC3-(4,5-dimethylthiazol-2-yl)-2,5-diphenyltetrazolium bromide - 3- (4،5-dimethylthiazol-2-yl) -2،5-difenyltetrazolium bromideMTT - MTTPulsed amperometric detector - آشکارساز آمپرترمتری پالس شدهHAEC - اینinterleukin 1β - اینترلوکین 1βcoronary artery disease - بیماری عروق کرونرDulbecco's minimal essential medium - حداقل کالای ضروری Dulbeccodegree of polymerization - درجه پلیمریزاسیونMolecular structure - ساختار مولکولیfetal bovine serum - سرم جنین گاوAortic endothelial cells - سلولهای اندوتلیال آئورتhuman aortic endothelial cells - سلولهای اندوتلیال آئورت انسانRefractive index - ضریب شکستCAD - طراحی به کمک رایانه یا کَدHuman epidermal growth factor - عامل رشد اپیدرمی انسانیHigh-performance anion-exchange chromatography - عملکرد بالا کروماتوگرافی مبدل آنیونیTNF-α - فاکتور نکروز توموری آلفاphosphate buffer saline - فسفات بافر شورAnti-inflammatory activity - فعالیت ضد التهابیendothelial growth medium - محیط رشد اندوتلیالendothelial cell basal medium - محیط پایه سلول اندوتلیالcell adhesion molecules - مولکول های چسبندگی سلولیvascular cell adhesion molecule - مولکول چسبندگی سلولی عروقیBars - میله هاMolecular weight - وزن مولکولیPAD - پدhigh performance size exclusion chromatography - کروماتوگرافی خروج با اندازه کافی بالاLDL-cholesterol - کلسترول LDLgastro-intestinal - گوارشی
موضوعات مرتبط
مهندسی و علوم پایه
شیمی
شیمی آلی
پیش نمایش صفحه اول مقاله
![عکس صفحه اول مقاله: Effect of oat and barley β-glucans on inhibition of cytokine-induced adhesion molecule expression in human aortic endothelial cells: Molecular structure-function relations Effect of oat and barley β-glucans on inhibition of cytokine-induced adhesion molecule expression in human aortic endothelial cells: Molecular structure-function relations](/preview/png/10604630.png)
چکیده انگلیسی
Endothelial cell adhesion molecules have been recognized as an early step in inflammation and atherogenesis. The inhibition of TNF-α-induced expression of vascular cell adhesion molecule (VCAM-1) and intracellular cell adhesion molecule (ICAM-1) in human aortic endothelial cells (HAEC), following pretreatment with mixed-linkage (1 â 3), (1 â 4)-β-d-glucans (β-glucans) isolated from oat and barley, and the possible molecular structure-function relations, have been explored. The apparent molecular weight (Mw) of β-glucans varied in the ranges of 0.71-2.42 Ã 105 (barley) and 0.36-2.55 Ã 105 (oat); a higher ratio of 3-O-β-cellobiosyl-d-glucose to 3-O-β-cellotriosyl-d-glucose oligomers in the polymeric chains was also found for barley β-glucans. Analysis of variance showed that polysaccharide concentration, Mw and fine structure of β-glucans affected the VCAM-1 expression (P < 0.05); the reduction of VCAM-1 expression in stimulated HAEC was also dose-dependent (concentration range of 3.75-200 μg/ml β-glucans) and significant at polysaccharide levels > 6.25 μg/ml (P < 0.05). The inhibition of VCAM-1 expression was greater for barley β-glucans compared to oat, displaying a maximum anti-inflammatory activity at Mw â¼1.40 Ã 105. Instead, the expression of ICAM-1 was suppressed (P < 0.05) only at high polysaccharide concentrations (>100 μg/ml), with maximum activity at Mw â¼2.50 Ã 105. Structural differences between the oat and barley β-glucans did to seem to influence the ICAM-1 expression.
ناشر
Database: Elsevier - ScienceDirect (ساینس دایرکت)
Journal: Carbohydrate Polymers - Volume 84, Issue 1, 11 February 2011, Pages 153-161
Journal: Carbohydrate Polymers - Volume 84, Issue 1, 11 February 2011, Pages 153-161
نویسندگان
Athina Lazaridou, Zoi Papoutsi, Costas G. Biliaderis, Paraskevi Moutsatsou,