کد مقاله | کد نشریه | سال انتشار | مقاله انگلیسی | نسخه تمام متن |
---|---|---|---|---|
10605786 | 982874 | 2010 | 12 صفحه PDF | دانلود رایگان |
عنوان انگلیسی مقاله ISI
A 3D-structural model of unsulfated chondroitin from high-field NMR: 4-sulfation has little effect on backbone conformation
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کلمات کلیدی
GAGHMBCGlcANOESYGalNAcCSPGHSQCN-acetyl-d-glucosamineGlcNAcPDBnuclear magnetic resonance - رزونانس مغناطیسی هستهایN-Acetyl-D-Galactosamine - N-استیل D-گالاکتوزامینNuclear Overhauser Effect SpectroscopY - Spectroscopy اثر Overhauser هسته ایd-Glucuronic acid - اسید d-گلوکورونیکheteronuclear multiple bond coherence - انسجام چندگانه چندگانه هترو هسته ایNMR - تشدید مغناطیسی هستهای Conformation - سازگاریcorrelation spectroscopy - طیف سنجی همبستگیHyaluronan - هیالورونانProteoglycan - پروتئوگلیکانProtein Data Bank - پروتئین بانک اطلاعاتیCOSY - کثیفChondroitin sulfate - کندرویتین سولفاتGlycosaminoglycan - گلیکوزآمینوگلیکانheteronuclear single quantum coherence - یکپارچگی کوانتومی تک هسته ای
موضوعات مرتبط
مهندسی و علوم پایه
شیمی
شیمی آلی
پیش نمایش صفحه اول مقاله
چکیده انگلیسی
The glycosaminoglycan chondroitin sulfate is essential in human health and disease but exactly how sulfation dictates its 3D-strucutre at the atomic level is unclear. To address this, we have purified homogenous oligosaccharides of unsulfated chondroitin (with and without 15N-enrichment) and analysed them by high-field NMR to make a comparison published chondroitin sulfate and hyaluronan 3D-structures. The result is the first full assignment of the tetrasaccharide and an experimental 3D-model of the hexasaccharide (PDB code 2KQO). In common with hyaluronan, we confirm that the amide proton is not involved in strong, persistent inter-residue hydrogen bonds. However, in contrast to hyaluronan, a hydrogen bond is not inferred between the hexosamine OH-4 and the glucuronic acid O5 atoms across the β(1â3) glycosidic linkage. The unsulfated chondroitin bond geometry differs slightly from hyaluronan by rotation about the β(1â3) Ï dihedral (as previously predicted by simulation), while the β(1â4) linkage is unaffected. Furthermore, comparison shows that this glycosidic linkage geometry is similar in chondroitin-4-sulfate. We therefore hypothesise that both hexosamine OH-4 and OH-6 atoms are solvent exposed in chondroitin, explaining why it is amenable to sulfation and hyaluronan is not, and also that 4-sulfation has little effect on backbone conformation. Our conclusions exemplify the value of the 3D-model presented here and progress our understanding of glycosaminoglycan molecular properties.
ناشر
Database: Elsevier - ScienceDirect (ساینس دایرکت)
Journal: Carbohydrate Research - Volume 345, Issue 2, 26 January 2010, Pages 291-302
Journal: Carbohydrate Research - Volume 345, Issue 2, 26 January 2010, Pages 291-302
نویسندگان
Benedict M. Sattelle, Javad Shakeri, Ian S. Roberts, Andrew Almond,