کد مقاله کد نشریه سال انتشار مقاله انگلیسی نسخه تمام متن
10691196 1019585 2016 7 صفحه PDF دانلود رایگان
عنوان انگلیسی مقاله ISI
Use of Hydroxyapatite Doping to Enhance Responsiveness of Heat-Inducible Gene Switches to Focused Ultrasound
ترجمه فارسی عنوان
استفاده از دوپینگ هیدروکسی آپاتیت برای افزایش پاسخگویی ژن های قابل تنظیم به حرارت به سونوگرافی متمرکز
کلمات کلیدی
سونوگرافی، هیپرترمی، هیدروژل، فیبرین، تضعیفی، ژن درمانی، هیدروکسی آپاتیت،
موضوعات مرتبط
مهندسی و علوم پایه فیزیک و نجوم آکوستیک و فرا صوت
چکیده انگلیسی
Recently, we demonstrated that ultrasound-based hyperthermia can activate cells containing a heat-activated and ligand-inducible gene switch in a spatio-temporally controlled manner. These engineered cells can be incorporated into hydrogel scaffolds (e.g., fibrin) for in vivo implantation, where ultrasound can be used to non-invasively pattern transgene expression. Due to their high water content, the acoustic attenuation of fibrin scaffolds is low. Thus, long ultrasound exposures and high acoustic intensities are needed to generate sufficient hyperthermia for gene activation. Here, we demonstrate that the attenuation of fibrin scaffolds and the resulting hyperthermia achievable with ultrasound can be increased significantly by doping the fibrin with hydroxyapatite (HA) nanopowder. The attenuation of a 1% (w/v) fibrin scaffold with 5% (w/v) HA was similar to soft tissue. Transgene activation of cells harboring the gene switch occurred at lower acoustic intensities and shorter exposures when the cells were encapsulated in HA-doped fibrin scaffolds versus undoped scaffolds. Inclusion of HA in the fibrin scaffold did not affect the viability of the encapsulated cells.
ناشر
Database: Elsevier - ScienceDirect (ساینس دایرکت)
Journal: Ultrasound in Medicine & Biology - Volume 42, Issue 3, March 2016, Pages 824-830
نویسندگان
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