کد مقاله | کد نشریه | سال انتشار | مقاله انگلیسی | نسخه تمام متن |
---|---|---|---|---|
10738834 | 1046836 | 2005 | 11 صفحه PDF | دانلود رایگان |
عنوان انگلیسی مقاله ISI
Hemoglobin autoxidation and regulation of endogenous H2O2 levels in erythrocytes
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کلمات کلیدی
3-ATPRXIAAGSHPxROS - ROSGene deletion - حذف ژنFree radicals - رادیکال آزادSOD - سدSuperoxide dismutase - سوکسوکس دیسموتازmetHb - مت هابmethemoglobin - متهموگلوبینHemoglobin - هموگلوبینPeroxiredoxin - پروکسی ردوکسینCatalase - کاتالازErythrocyte - گلبول قرمز یا اریتروسیتGlutathione - گلوتاتیونglutathione peroxidase - گلوتاتیون پراکسیدازReactive oxygen species - گونههای فعال اکسیژنiodoacetamide - یووداکتامید
موضوعات مرتبط
علوم زیستی و بیوفناوری
بیوشیمی، ژنتیک و زیست شناسی مولکولی
سالمندی
پیش نمایش صفحه اول مقاله
چکیده انگلیسی
Red cells from mice deficient in glutathione peroxidase-1 were used to estimate the hemoglobin autoxidation rate and the endogenous level of H2O2 and superoxide. Methemoglobin and the rate of catalase inactivation by 3-amino-2,4,5-triazole (3-AT) were determined. In contrast with iodoacetamide-treated red cells, catalase was not inactivated by 3-AT in glutathione peroxidase-deficient erythrocytes. Kinetic models incorporating reactions known to involve H2O2 and superoxide in the erythrocyte were used to estimate H2O2, superoxide, and methemoglobin levels. The experimental data could not be modeled unless the intraerythrocytic concentration of Compound I is very low. Two additional models were tested. In one, it was assumed that a rearranged Compound I, termed Compound II*, does not react with 3-AT. However, experiments with an NADPH-generating system provided evidence that this mechanism does not occur. A second model that explicitly includes peroxiredoxin II can fit the experimental findings. Insertion of the data into the model predicted a hemoglobin autoxidation rate constant of 4.5Â ÃÂ 10â7 sâ1 and an endogenous H2O2 and superoxide concentrations of 5Â ÃÂ 10â11 and 5Â ÃÂ 10â13 M, respectively, lower than previous estimates.
ناشر
Database: Elsevier - ScienceDirect (ساینس دایرکت)
Journal: Free Radical Biology and Medicine - Volume 39, Issue 11, 1 December 2005, Pages 1407-1417
Journal: Free Radical Biology and Medicine - Volume 39, Issue 11, 1 December 2005, Pages 1407-1417
نویسندگان
Robert M. Johnson, Gerard Jr., Y. Ravindranath, Ye-Shih Ho,