کد مقاله | کد نشریه | سال انتشار | مقاله انگلیسی | نسخه تمام متن |
---|---|---|---|---|
10739344 | 1046872 | 2005 | 18 صفحه PDF | دانلود رایگان |
عنوان انگلیسی مقاله ISI
Determining the oxidation states of manganese in PC12 and nerve growth factor-induced PC12 cells
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کلمات کلیدی
PHPAXANESTPPtetraphenylphosphoniumtBHPNGFnuclear magnetic resonance - رزونانس مغناطیسی هستهایROS - ROSNMR - تشدید مغناطیسی هستهای Electron paramagnetic resonance - تشدید پارامغناطیس الکترونEPR - تشدید پارامغناطیس الکترونX-ray absorption near-edge structure - جذب اشعه ایکس ساختار نزدیک به لبهSOD - سدPC12 cells - سلول های PC12Manganese toxicity - سمیت منگنزSuperoxide dismutase - سوکسوکس دیسموتازXANES spectroscopy - طیف سنجی XANESnerve growth factor - فاکتور رشد عصبtert-butyl hydroperoxide - هیدروپراکسید تری بوتیلReactive oxygen species - گونههای فعال اکسیژن
موضوعات مرتبط
علوم زیستی و بیوفناوری
بیوشیمی، ژنتیک و زیست شناسی مولکولی
سالمندی
پیش نمایش صفحه اول مقاله
![عکس صفحه اول مقاله: Determining the oxidation states of manganese in PC12 and nerve growth factor-induced PC12 cells Determining the oxidation states of manganese in PC12 and nerve growth factor-induced PC12 cells](/preview/png/10739344.png)
چکیده انگلیسی
Excessive brain Mn can produce toxicity with symptoms resembling parkinsonism. This syndrome, called “manganism,” correlates with loss of dopamine in the striatum and cell death in the striatum and globus pallidus. A common hypothesis is that cell damage in Mn toxicity is caused by oxidation of important cell components by Mn3+. Determination of the amount of Mn3+ present, under a range of conditions, in neuronal cells and brain mitochondria represents an important step in evaluating the “damage through oxidation by Mn3+ hypothesis.” In an earlier paper we used X-ray absorption near-edge structure (XANES) spectroscopy to determine the amount of Mn2+ and Mn3+ in brain mitochondria under a range of conditions. Here we extend the study to investigate the evidence for formation of Mn3+ through oxidation of Mn2+ by ROS in PC12 cells and in PC12 cells induced with nerve growth factor (NGF) to display a phenotype more like that of neurons. Although the results suggest that very small amounts of Mn3+ might be present at low Mn levels, probably in Mn superoxide dismutase, Mn3+ is not stabilized by complex formation in these cells and therefore does not accumulate to detectable amounts.
ناشر
Database: Elsevier - ScienceDirect (ساینس دایرکت)
Journal: Free Radical Biology and Medicine - Volume 39, Issue 2, 15 July 2005, Pages 164-181
Journal: Free Radical Biology and Medicine - Volume 39, Issue 2, 15 July 2005, Pages 164-181
نویسندگان
Karlene K. Gunter, Michael Aschner, Lisa M. Miller, Roman Eliseev, Jason Salter, Katie Anderson, Sean Hammond, Thomas E. Gunter,