کد مقاله | کد نشریه | سال انتشار | مقاله انگلیسی | نسخه تمام متن |
---|---|---|---|---|
10748545 | 1050275 | 2016 | 6 صفحه PDF | دانلود رایگان |
عنوان انگلیسی مقاله ISI
Laminin 411 and 511 promote the cholangiocyte differentiation of human induced pluripotent stem cells
ترجمه فارسی عنوان
لامینین 411 و 511 ترویج تمایز کلنگیوسیت سلول های بنیادی پلورپوفنت القا شده توسط انسان
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کلمات کلیدی
EGFSRY-box 9G protein-coupled bile acid receptor 1SCTRJAG1Jagged 1GPBAR1CholangiocyteAQP1HBCHGFIL6CFTRFGFSox9OSMFBSBSA - BSAiPS - IPSγ-Glutamyl transferase - γ-گلوتامیل ترانسفرازbovine serum albumin - آلبومین سرم گاوaquaporin 1 - آکوپورین 1oncostatin M - اوکتواستاتین مinterleukin 6 - اینترلوکین 6cystic fibrosis transmembrane conductance regulator - رگولاتور رسانایی فرابنفش فیبروز کیستیکinduced pluripotent stem - ساقه پلورپوفنت القا شده استfetal bovine serum - سرم جنین گاوHuman induced pluripotent stem cells - سلول های بنیادی تکامل یافته القا شده توسط انسانepidermal growth factor - عامل رشد اپیدرمیHepatocyte growth factor - عامل رشد هپاتوسیتfibroblast growth factor - فاکتور رشد فیبروبلاستLaminin - لامینینBMP - مدیریت فرایند کسب و کارBone morphogenetic protein - پروتئین مورفوژنیک استخوانSecretin receptor - گیرنده سکترون
موضوعات مرتبط
علوم زیستی و بیوفناوری
بیوشیمی، ژنتیک و زیست شناسی مولکولی
زیست شیمی
چکیده انگلیسی
The drug discovery research for cholestatic liver diseases has been hampered by the lack of a well-established human cholangiocyte model. Functional cholangiocyte-like cells differentiated from human induced pluripotent stem (iPS) cells are expected to be a promising candidate for such research, but there remains no well-established method for differentiating cholangiocytes from human iPS cells. In this study, we searched for a suitable extracellular matrix to promote cholangiocyte differentiation from human iPS cells, and found that both laminin 411 and laminin 511 were suitable for this purpose. The gene expression levels of the cholangiocyte markers, aquaporin 1 (AQP1), SRY-box 9 (SOX9), cystic fibrosis transmembrane conductance regulator (CFTR), G protein-coupled bile acid receptor 1 (GPBAR1), Jagged 1 (JAG1), secretin receptor (SCTR), and γ-glutamyl transferase (GGT1) were increased by using laminin 411 or laminin 511 as a matrix. In addition, the percentage of AQP1-positive cells was increased from 61.8% to 92.5% by using laminin 411 or laminin 511. Furthermore, the diameter and number of cysts consisted of cholangiocyte-like cells were increased when using either matrix. We believe that the human iPS cell-derived cholangiocyte-like cells, which were generated by using our differentiation technology, would be useful for the drug discovery research of cholestatic liver diseases.
ناشر
Database: Elsevier - ScienceDirect (ساینس دایرکت)
Journal: Biochemical and Biophysical Research Communications - Volume 474, Issue 1, 20 May 2016, Pages 91-96
Journal: Biochemical and Biophysical Research Communications - Volume 474, Issue 1, 20 May 2016, Pages 91-96
نویسندگان
Kazuo Takayama, Seiji Mitani, Yasuhito Nagamoto, Fuminori Sakurai, Masashi Tachibana, Yukimasa Taniguchi, Kiyotoshi Sekiguchi, Hiroyuki Mizuguchi,