کد مقاله | کد نشریه | سال انتشار | مقاله انگلیسی | نسخه تمام متن |
---|---|---|---|---|
10749109 | 1050286 | 2015 | 6 صفحه PDF | دانلود رایگان |
عنوان انگلیسی مقاله ISI
24-Methylenecycloartanyl ferulate, a major compound of γ-oryzanol, promotes parvin-beta expression through an interaction with peroxisome proliferator-activated receptor-gamma 2 in human breast cancer cells
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کلمات کلیدی
PPAREMSAPPAR-γ2Akt1GSKCAFGSTEGFPBSDMEMFBSDMSO - DMSODulbecco's modified Eagle's medium - Medal of Eagle اصلاح شده DulbeccoElectrophoretic mobility shift assay - آزمون تحرک تحرک الکتروفورزPPRE - ارسالILK - اولchromatin immunoprecipitation - ایمن سازی کروماتینDimethyl sulfoxide - دیمتیل سولفواکسیدreverse transcription - رونویسی معکوسfetal bovine serum - سرم جنین گاوepidermal growth factor - عامل رشد اپیدرمیperoxisome proliferator response element - عنصر پاسخ پرولیفراتور پروکسیومFerulic acid - فرولیک اسیدCSF - مایع مغزی نخاعیPhosphate-buffered saline - محلول نمک فسفات با خاصیت بافریcalponin homology - همولوگ با کالپونCHiP - چیپintegrin-linked kinase - کیناز مرتبط با انتگرالglutathione S-transferase - گلوتاتیون S-ترانسفرازglycogen synthase kinase - گلیکوزین سیتستاز کینازperoxisome proliferator-activated receptor - گیرنده فعال فعال پروکسیوم
موضوعات مرتبط
علوم زیستی و بیوفناوری
بیوشیمی، ژنتیک و زیست شناسی مولکولی
زیست شیمی
پیش نمایش صفحه اول مقاله
![عکس صفحه اول مقاله: 24-Methylenecycloartanyl ferulate, a major compound of γ-oryzanol, promotes parvin-beta expression through an interaction with peroxisome proliferator-activated receptor-gamma 2 in human breast cancer cells 24-Methylenecycloartanyl ferulate, a major compound of γ-oryzanol, promotes parvin-beta expression through an interaction with peroxisome proliferator-activated receptor-gamma 2 in human breast cancer cells](/preview/png/10749109.png)
چکیده انگلیسی
Parvin-β is an adaptor protein that binds to integrin-linked kinase (ILK) and is significantly downregulated in breast tumors and breast cancer cell lines. We treated the breast cancer cell line MCF7 with 24-methylenecycloartanyl ferulate (24-MCF), a γ-oryzanol compound. We observed upregulation of parvin-β (GenBank Accession No. AF237769) and peroxisome proliferator-activated receptor (PPAR)-γ2 (GenBank Accession No. NM_015869). Among γ-oryzanol compounds, only treatment with 24-MCF led to the formation of reverse transcription-PCR products of parvin-β (650 and 500 bp) and PPAR-γ2 (580 bp) in MCF7 cells, but not in T47D, SK-BR-3, or MDA-MB-231 cells. 24-MCF treatment increased the mRNA and protein levels of parvin-β in MCF7 cells in a dose-dependent manner. We hypothesized that there is a correlation between parvin-β expression and induction of PPAR-γ2. This hypothesis was investigated by using a promoter-reporter assay, chromatin immunoprecipitation, and an electrophoretic mobility shift assay. 24-MCF treatment induced binding of PPAR-γ2 to a peroxisome proliferator response element-like cis-element (ACTAGGACAAAGGACA) in the parvin-β promoter in MCF7 cells in a dose-dependent manner. 24-MCF treatment significantly decreased anchorage-independent growth and inhibited cell movement in comparison to control treatment with dimethyl sulfoxide. 24-MCF treatment reduced the levels of GTP-bound Rac1 and Cdc42. Evaluation of Akt1 inhibition by 24-MCF revealed that the half maximal effective concentration was 33.3 μM. Docking evaluations revealed that 24-MCF binds to the ATP-binding site of Akt1(PDB ID: 3OCB) and the compound binding energy is -8.870 kcal/mol. Taken together, our results indicate that 24-MCF treatment increases parvin-β expression, which may inhibit ILK downstream signaling.
ناشر
Database: Elsevier - ScienceDirect (ساینس دایرکت)
Journal: Biochemical and Biophysical Research Communications - Volume 468, Issue 4, 25 December 2015, Pages 574-579
Journal: Biochemical and Biophysical Research Communications - Volume 468, Issue 4, 25 December 2015, Pages 574-579
نویسندگان
Heon Woong Kim, Eun Joung Lim, Hwan Hee Jang, XueLei Cui, Da Rae Kang, Sung Hyen Lee, Haeng Ran Kim, Jeong Sook Choe, Young Mok Yang, Jung Bong Kim, Jong Hwan Park,