کد مقاله | کد نشریه | سال انتشار | مقاله انگلیسی | نسخه تمام متن |
---|---|---|---|---|
10750747 | 1050302 | 2015 | 7 صفحه PDF | دانلود رایگان |
عنوان انگلیسی مقاله ISI
Different effects of resveratrol on early and late passage mesenchymal stem cells through β-catenin regulation
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کلمات کلیدی
Sirt1GSK-3βDAPISA-β-galTCFqRT-PCRERKMSCCFU-Fsirtuin 1FBSDMEM-LGPBS4′,6-diamidino-2-phenylindole - 4 '، 6-دیامیدینو-2-فنیلینولβ-catenin - بتا-کاتنینResveratrol - رسوراترولfetal bovine serum - سرم جنین گاوMesenchymal stem cells - سلول های بنیادی مزانشیمیMesenchymal stem cell - سلول های بنیادی مزانشیمیT cell factor - عامل سلول TPhosphate buffered saline - فسفات بافر شورQuantitative reverse transcriptase polymerase chain reaction - واکنش زنجیره ای پلی مراز ترانس کریتاز معکوس کمیsenescence-associated-β-galactosidase - پیری زودرس، بتا گالاکتوزیدازCellular senescence - پیری سلولیextracellular signal-related kinase - کیناز مرتبط با سیگنال خارج سلولیGlycogen synthase kinase 3β - گلیکوزین سنتاز کیناز 3β
موضوعات مرتبط
علوم زیستی و بیوفناوری
بیوشیمی، ژنتیک و زیست شناسی مولکولی
زیست شیمی
پیش نمایش صفحه اول مقاله
![عکس صفحه اول مقاله: Different effects of resveratrol on early and late passage mesenchymal stem cells through β-catenin regulation Different effects of resveratrol on early and late passage mesenchymal stem cells through β-catenin regulation](/preview/png/10750747.png)
چکیده انگلیسی
Resveratrol is a sirtuin 1 (SIRT1) activator and can function as an anti-inflammatory and antioxidant factor. In mesenchymal stem cells (MSCs), resveratrol enhances the proliferation and differentiation potential and has an anti-aging effect. However, contradictory effects of resveratrol on MSC cultures have been reported. In this study, we found that resveratrol had different effects on MSC cultures according to their cell passage and SIRT1 expression. Resveratrol enhanced the self-renewal potential and multipotency of early passage MSCs, but accelerated cellular senescence of late passage MSCs. In early passage MSCs expressing SIRT1, resveratrol decreased ERK and GSK-3β phosphorylation, suppressing β-catenin activity. In contrast, in late passage MSCs, which did not express SIRT1, resveratrol increased ERK and GSK-3β phosphorylation, activating β-catenin. We confirmed that SIRT1-deficient early passage MSCs treated with resveratrol lost their self-renewal potential and multipotency, and became senescent due to increased β-catenin activity. Sustained treatment with resveratrol at early passages maintained the self-renewal potential and multipotency of MSCs up to passage 10. Our findings suggest that resveratrol can be effectively applied to early passage MSC cultures, whereas parameters such as cell passage and SIRT1 expression must be taken into consideration before applying resveratrol to late passage MSCs.
ناشر
Database: Elsevier - ScienceDirect (ساینس دایرکت)
Journal: Biochemical and Biophysical Research Communications - Volume 467, Issue 4, 27 November 2015, Pages 1026-1032
Journal: Biochemical and Biophysical Research Communications - Volume 467, Issue 4, 27 November 2015, Pages 1026-1032
نویسندگان
Dong Suk Yoon, Yoorim Choi, Seong Mi Choi, Kwang Hwan Park, Jin Woo Lee,