کد مقاله | کد نشریه | سال انتشار | مقاله انگلیسی | نسخه تمام متن |
---|---|---|---|---|
10752018 | 1050322 | 2015 | 7 صفحه PDF | دانلود رایگان |
عنوان انگلیسی مقاله ISI
Fluid shear stress promotes proprotein convertase-dependent activation of MT1-MMP
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کلمات کلیدی
ECMWSSMT1-MMPS1PThree-dimensionalGFPHUVECGAPDHFGFECs - EC هاAngiogenesis - آنژیوژنزWall shear stress - استرس برش دیوارsphingosine 1-phosphate - اسپینگزین 1-فسفاتSprouting - جوانه زدنHuman umbilical vein endothelial cell - سلول اندوتلیالی ورید ناقل انسانیEndothelial cells - سلولهای اندوتلیالVascular endothelial growth factor - فاکتور رشد اندوتلیال عروقیVascular Endothelial Growth Factor (VEGF) - فاکتور رشد اندوتلیال عروقی (VEGF)fibroblast growth factor - فاکتور رشد فیبروبلاستFurin - فورینExtracellular matrix - ماتریکس خارج سلولیmembrane type-1 matrix metalloproteinase - متالوپروتئیناز ماتریکس نوع 1 غشاییMechanotransduction - مکانیک انتقالgreen fluorescent protein - پروتئین فلورسنت سبزproprotein convertase - پروپروتئین تبدیل کنندهGlyceraldehyde phosphate dehydrogenase - گلیسرالیدید فسفات دهیدروژناز
موضوعات مرتبط
علوم زیستی و بیوفناوری
بیوشیمی، ژنتیک و زیست شناسی مولکولی
زیست شیمی
پیش نمایش صفحه اول مقاله
![عکس صفحه اول مقاله: Fluid shear stress promotes proprotein convertase-dependent activation of MT1-MMP Fluid shear stress promotes proprotein convertase-dependent activation of MT1-MMP](/preview/png/10752018.png)
چکیده انگلیسی
During angiogenesis, endothelial cells (ECs1) initiate new blood vessel growth and invade into the extracellular matrix (ECM). Membrane type-1 matrix metalloproteinase (MT1-MMP) facilitates this process and translocates to the plasma membrane following activation to promote ECM cleavage. The N-terminal pro-domain within MT1-MMP must be processed for complete activity of the proteinase. This study investigated whether MT1-MMP activation was altered by sphingosine 1-phosphate (S1P) and wall shear stress (WSS), which combine to stimulate EC invasion in three dimensional (3D) collagen matrices. MT1-MMP was activated rapidly and completely by WSS but not S1P. Proprotein convertases (PCs) promoted MT1-MMP processing, prompting us to test whether WSS or S1P treatments increased PC activity. Like MT1-MMP, PC activity increased with WSS, while S1P had no effect. A pharmacological PC inhibitor completely blocked S1P- and WSS-induced EC invasion and MT1-MMP translocation to the plasma membrane. Further, a recombinant PC inhibitor reduced MT1-MMP activation and decreased lumen formation in invading ECs, a process known to be controlled by MT1-MMP. Thus, we conclude that PC and MT1-MMP activation are mechanosensitive events that are required for EC invasion into 3D collagen matrices.
ناشر
Database: Elsevier - ScienceDirect (ساینس دایرکت)
Journal: Biochemical and Biophysical Research Communications - Volume 460, Issue 3, 8 May 2015, Pages 596-602
Journal: Biochemical and Biophysical Research Communications - Volume 460, Issue 3, 8 May 2015, Pages 596-602
نویسندگان
Hojin Kang, Camille L. Duran, Colette A. Abbey, Roland R. Kaunas, Kayla J. Bayless,