کد مقاله | کد نشریه | سال انتشار | مقاله انگلیسی | نسخه تمام متن |
---|---|---|---|---|
10752757 | 1050331 | 2015 | 26 صفحه PDF | دانلود رایگان |
عنوان انگلیسی مقاله ISI
Peroxisome proliferator-activated receptor-γ (PPAR-γ) agonist inhibits collagen synthesis in human hypertrophic scar fibroblasts by targeting Smad3 via miR-145
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کلمات کلیدی
ECMPPAR-γRT-PCRSmad3PPAR-γ agonistUTRMiR-145 - MIR-145reverse transcriptase-PCR - RT-PCR معکوسFibrosis - فیبروز یا فساد الیافExtracellular matrix - ماتریکس خارج سلولیuntranslated region - منطقه غیر ترجمهMicroRNA - میکرو RNA MiRNA - میکروRNA، ریزآرانای، miRNACol1 - کل 1Collagen type I - کلاژن نوع IPeroxisome proliferator-activated receptor-γ - گیرنده پروتئینی فعال پروکسیوم - γ
موضوعات مرتبط
علوم زیستی و بیوفناوری
بیوشیمی، ژنتیک و زیست شناسی مولکولی
زیست شیمی
پیش نمایش صفحه اول مقاله
چکیده انگلیسی
The transcription factor peroxisome proliferator-activated receptor-γ (PPAR-γ) functions to regulate cell differentiation and lipid metabolism. Recently, its agonist has been documented to regulate extracellular matrix production in human dermal fibroblasts. This study explored the underlying molecular mechanisms and gene interactions in hypertrophic scar fibroblasts (HSFBs) in vitro. HSFBs were cultured and treated with or without PPAR-γ agonist or antagonist for gene expression. Bioinformatical analysis predicted that miR-145 could target Smad3 expression. Luciferase assay was used to confirm such an interaction. The data showed that PPAR-γ agonist troglitazone suppressed expression of Smad3 and Col1 in HSFBs. PPAR-γ agonist induced miR-145 at the gene transcriptional level, which in turn inhibited Smad3 expression and Col1 level in HSFBs. Furthermore, ELISA data showed that Col1 level in HSFBs was controlled by a feedback regulation mechanism involved in PPAR-γ agonist and antagonist-regulated expression of miR-145 and Smad3 in HSFBs. These findings indicate that PPAR-γ-miR-145-Smad3 axis plays a role in regulation of collagen synthesis in HSFBs.
ناشر
Database: Elsevier - ScienceDirect (ساینس دایرکت)
Journal: Biochemical and Biophysical Research Communications - Volume 459, Issue 1, 27 March 2015, Pages 49-53
Journal: Biochemical and Biophysical Research Communications - Volume 459, Issue 1, 27 March 2015, Pages 49-53
نویسندگان
Hua-Yu Zhu, Chao Li, Zhao Zheng, Qin Zhou, Hao Guan, Lin-Lin Su, Jun-Tao Han, Xiong-Xiang Zhu, Shu-yue Wang, Jun Li, Da-Hai Hu,