کد مقاله | کد نشریه | سال انتشار | مقاله انگلیسی | نسخه تمام متن |
---|---|---|---|---|
10752808 | 1050332 | 2015 | 6 صفحه PDF | دانلود رایگان |
عنوان انگلیسی مقاله ISI
Effective internalization of U251-MG-secreted exosomes into cancer cells and characterization of their lipid components
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کلمات کلیدی
MDAU251DLSMDA-MB-231GBMAFMDICTLCAstrocyte - آستروسیتAST - آسپارتات ترانس آمینازsphingomyelin - اسفنگومیلینExosomes - اگزوزوم هاDrug delivery system - سیستم تحویل داروphosphatidylethanolamine - فسفاتیدیلتانولامینLipid - لیپیدatomic force microscopy - میکروسکوپ نیروی اتمیCancer cell targeting - هدف سلول سرطانیextracellular vesicle - ویسکوز خارج سلولیDynamic Light Scattering - پراکندگی نور دینامیکیthin-layer chromatography - کروماتوگرافی نازک لایهdifferential interference contrast - کنتراست تداخل دیفرانسیلGlioblastoma - گلیوبلاستوما
موضوعات مرتبط
علوم زیستی و بیوفناوری
بیوشیمی، ژنتیک و زیست شناسی مولکولی
زیست شیمی
پیش نمایش صفحه اول مقاله
چکیده انگلیسی
Exosomes, the natural vehicles of various biological molecules, have been examined in several research fields including drug delivery. Although understanding of the biological functions of exosomes has increased, how exosomes are transported between cells remains unclear. We hypothesized that cell tropism is important for effective exosomal intercellular communication and that parental cells regulate exosome movement by modulating constituent exosomal molecules. Herein, we demonstrated the strong translocation of glioblastoma-derived exosomes (U251exo) into their parental (U251) cells, breast cancer (MDA-MB-231) cells, and fibrosarcoma (HT-1080). Furthermore, disruption of proteins of U251exo by enzymatic treatment did not affect their uptake. Therefore, we focused on lipid molecules of U251exo with the expectation that they are crucial for effective incorporation of U251exo by cancer cells. Phosphatidylethanolamine was identified as a unique lipid component of U251-MG cell-derived extracellular vesicles. From these results, valuable insight is provided into the targeting of U251exo to cancer cells, which will help to develop a cancer-targeted drug delivery system.
ناشر
Database: Elsevier - ScienceDirect (ساینس دایرکت)
Journal: Biochemical and Biophysical Research Communications - Volume 456, Issue 3, 16 January 2015, Pages 768-773
Journal: Biochemical and Biophysical Research Communications - Volume 456, Issue 3, 16 January 2015, Pages 768-773
نویسندگان
Yuki Toda, Kazuyuki Takata, Yuko Nakagawa, Hikaru Kawakami, Shusuke Fujioka, Kazuya Kobayashi, Yasunao Hattori, Yoshihisa Kitamura, Kenichi Akaji, Eishi Ashihara,