کد مقاله | کد نشریه | سال انتشار | مقاله انگلیسی | نسخه تمام متن |
---|---|---|---|---|
10752844 | 1050333 | 2015 | 7 صفحه PDF | دانلود رایگان |
عنوان انگلیسی مقاله ISI
Redox sensor proteins for highly sensitive direct imaging of intracellular redox state
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کلمات کلیدی
BFPcyan fluorescence proteinGRX1roGFPYFPCFPGSSGAMSFörster resonance energy transfer - انتقال انرژی تابشی ForsterFRET - انتقال انرژی رزونانسی فورسترBiosensor - بیوسنسورDisulfide - دی سولفیدendoplasmic reticulum - شبکه آندوپلاسمی Fluorescence - فلورسنسRedox regulation - مقررات RedoxYellow Fluorescence Protein - پروتئین فلورسانس زردoxidation-reduction - کاهش اکسیداسیونoxidized glutathione - گلوتاتیون اکسید شده
موضوعات مرتبط
علوم زیستی و بیوفناوری
بیوشیمی، ژنتیک و زیست شناسی مولکولی
زیست شیمی
پیش نمایش صفحه اول مقاله
چکیده انگلیسی
Intracellular redox state is a critical factor for fundamental cellular functions, including regulation of the activities of various metabolic enzymes as well as ROS production and elimination. Genetically-encoded fluorescent redox sensors, such as roGFP (Hanson, G. T., et al. (2004)) and Redoxfluor (Yano, T., et al. (2010)), have been developed to investigate the redox state of living cells. However, these sensors are not useful in cells that contain, for example, other colored pigments. We therefore intended to obtain simpler redox sensor proteins, and have developed oxidation-sensitive fluorescent proteins called Oba-Q (oxidation balance sensed quenching) proteins. Our sensor proteins derived from CFP and Sirius can be used to monitor the intracellular redox state as their fluorescence is drastically quenched upon oxidation. These blue-shifted spectra of the Oba-Q proteins enable us to monitor various redox states in conjunction with other sensor proteins.
ناشر
Database: Elsevier - ScienceDirect (ساینس دایرکت)
Journal: Biochemical and Biophysical Research Communications - Volume 457, Issue 3, 13 February 2015, Pages 242-248
Journal: Biochemical and Biophysical Research Communications - Volume 457, Issue 3, 13 February 2015, Pages 242-248
نویسندگان
Kazunori Sugiura, Takeharu Nagai, Masahiro Nakano, Hiroshi Ichinose, Takakazu Nakabayashi, Nobuhiro Ohta, Toru Hisabori,