کد مقاله | کد نشریه | سال انتشار | مقاله انگلیسی | نسخه تمام متن |
---|---|---|---|---|
10753382 | 1050340 | 2014 | 6 صفحه PDF | دانلود رایگان |
عنوان انگلیسی مقاله ISI
Targeting miR-21 sensitizes Ph+ ALL Sup-b15 cells to imatinib-induced apoptosis through upregulation of PTEN
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کلمات کلیدی
qRT-PCRCMLImatinib - ایماتینیبmiR-21 - به miR-21Apoptosis - خزان یاختهایAcute lymphoblastic leukaemia - لوسمی لنفوبلاستی حادchronic myelogenous leukemia - لوسمی مزمن میلوئیدیMicroRNA - میکرو RNA MiRNA - میکروRNA، ریزآرانای، miRNAALL - همهquantitative real-time PCR - واکنش زنجیره ای پلیمراز واقعی در زمان واقعیPten - ژن PTEN
موضوعات مرتبط
علوم زیستی و بیوفناوری
بیوشیمی، ژنتیک و زیست شناسی مولکولی
زیست شیمی
پیش نمایش صفحه اول مقاله
![عکس صفحه اول مقاله: Targeting miR-21 sensitizes Ph+ ALL Sup-b15 cells to imatinib-induced apoptosis through upregulation of PTEN Targeting miR-21 sensitizes Ph+ ALL Sup-b15 cells to imatinib-induced apoptosis through upregulation of PTEN](/preview/png/10753382.png)
چکیده انگلیسی
Philadelphia chromosome positive (Ph+) acute lymphoblastic leukemia (ALL) cells are insensitive to BCR-ABL tyrosine kinase inhibitor imatinib, the underlying mechanisms remain largely unknown. Here, we showed that imatinib treatment induced significant upregulation of miR-21 and downregulation of PTEN in Ph+ ALL cell line Sup-b15. Transient inhibition of miR-21 resulted in increased apoptosis, PTEN upregulation and AKT dephosphorylation, whereas ectopic overexpression of miR-21 further conferred imatinib resistance. Furthermore, knockdown of PTEN protected the cells from imatinib-induced apoptosis achieved by inhibition of miR-21. Additionally, PI3K inhibitors also notably enhanced the effects of imatinib on Sup-b15 cells and primary Ph+ ALL cells similar to miR-21 inhibitor. Therefore, miR-21 contributes to imatinib resistance in Ph+ ALL cells and antagonizing miR-21 demonstrates therapeutic potential by sensitizing the malignancy to imatinib therapy.
ناشر
Database: Elsevier - ScienceDirect (ساینس دایرکت)
Journal: Biochemical and Biophysical Research Communications - Volume 454, Issue 3, 21 November 2014, Pages 423-428
Journal: Biochemical and Biophysical Research Communications - Volume 454, Issue 3, 21 November 2014, Pages 423-428
نویسندگان
Wei-Zhang Wang, Xiang-Hua Lin, Qiao-Hong Pu, Man-Yu Liu, Li Li, Li-Rong Wu, Qing-Qing Wu, Jian-Wen Mao, Jia-Yong Zhu, Xiao-Bao Jin,