کد مقاله کد نشریه سال انتشار مقاله انگلیسی نسخه تمام متن
10755101 1050367 2014 6 صفحه PDF دانلود رایگان
عنوان انگلیسی مقاله ISI
Identification of hepatitis B virus-specific CTL epitopes presented by HLA-A∗33:03 in peripheral blood mononuclear cells from patients and transgenic mice
موضوعات مرتبط
علوم زیستی و بیوفناوری بیوشیمی، ژنتیک و زیست شناسی مولکولی زیست شیمی
پیش نمایش صفحه اول مقاله
Identification of hepatitis B virus-specific CTL epitopes presented by HLA-A∗33:03 in peripheral blood mononuclear cells from patients and transgenic mice
چکیده انگلیسی
Cytotoxic T lymphocyte (CTL) epitopes in the HBV protein of hepatitis B virus (HBV) may play a key role in viral control and liver damage. The aim of this study was to identify and study the function of HLA-A∗33:03-restricted CTL epitopes in HBV protein of the HBV genotypes B and C, which are epidemic in China. Sixteen HBV peptides were predicated by computational analysis, and synthesized peptides were examined for their affinity to HLA-A∗33:03 using a stable cell line. After being analyzed by enzyme-linked immunospot and cytolytic activity assays, as well as the tetramers staining method using peripheral blood mononuclear cells isolated from HBV-infected patients, five peptides (Hbs245-253, HBs335-343, HBc119-127, HBc104-112, and HBp391-399) were chosen to further confirm their HLA_A∗33:03 restriction in transgenic mice.
ناشر
Database: Elsevier - ScienceDirect (ساینس دایرکت)
Journal: Biochemical and Biophysical Research Communications - Volume 449, Issue 1, 20 June 2014, Pages 135-140
نویسندگان
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