کد مقاله | کد نشریه | سال انتشار | مقاله انگلیسی | نسخه تمام متن |
---|---|---|---|---|
10755840 | 1050378 | 2014 | 6 صفحه PDF | دانلود رایگان |
عنوان انگلیسی مقاله ISI
Ascorbate reverses high glucose- and RAGE-induced leak of the endothelial permeability barrier
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کلمات کلیدی
Krebs-Ringer HEPESHEPESNACKRHSVCT2RAGEHMGB1N-2-hydroxyethylpiperazine-N′-2-ethanesulfonic acid - N-2-hydroxyethylpiperazine-N'-2-ethanesulfonic acidN-acetylcysteine - N-استیل سیستئینAscorbate - آسکوربات یا ویتامین ثOxidative stress - تنش اکسیداتیوAge - سنadvanced glycation end-products - محصولات نهایی گلیسایی پیشرفتهEndothelial permeability - نفوذ پذیری اندوتلیالHigh mobility group box 1 - کادر تحرک بالا 1high glucose - گلوکز بالا یا قند بالاReceptor for advanced glycation end-products - گیرنده برای پایان دادن به محصولات glycation پیشرفته
موضوعات مرتبط
علوم زیستی و بیوفناوری
بیوشیمی، ژنتیک و زیست شناسی مولکولی
زیست شیمی
پیش نمایش صفحه اول مقاله
چکیده انگلیسی
High glucose concentrations due to diabetes increase leakage of plasma constituents across the endothelial permeability barrier. We sought to determine whether vitamin C, or ascorbic acid (ascorbate), could reverse such high glucose-induced increases in endothelial barrier permeability. Human umbilical vein endothelial cells and two brain endothelial cell lines cultured at 25Â mM glucose showed increases in endothelial barrier permeability to radiolabeled inulin compared to cells cultured at 5Â mM glucose. Acute loading of the cells for 30-60Â min with ascorbate before the permeability assay prevented the high glucose-induced increase in permeability and decreased basal permeability at 5Â mM glucose. High glucose-induced barrier leakage was mediated largely by activation of the receptor for advanced glycation end products (RAGE), since it was prevented by RAGE blockade and mimicked by RAGE ligands. Intracellular ascorbate completely prevented RAGE ligand-induced increases in barrier permeability. The high glucose-induced increase in endothelial barrier permeability was also acutely decreased by several cell-penetrant antioxidants, suggesting that at least part of the ascorbate effect could be due to its ability to act as an antioxidant.
ناشر
Database: Elsevier - ScienceDirect (ساینس دایرکت)
Journal: Biochemical and Biophysical Research Communications - Volume 445, Issue 1, 28 February 2014, Pages 30-35
Journal: Biochemical and Biophysical Research Communications - Volume 445, Issue 1, 28 February 2014, Pages 30-35
نویسندگان
M. Elizabeth Meredith, Zhi-Chao Qu, James M. May,