کد مقاله | کد نشریه | سال انتشار | مقاله انگلیسی | نسخه تمام متن |
---|---|---|---|---|
10755972 | 1050379 | 2014 | 7 صفحه PDF | دانلود رایگان |
عنوان انگلیسی مقاله ISI
Monitoring of the serum proteome in Kawasaki disease patients before and after immunoglobulin therapy
ترجمه فارسی عنوان
نظارت بر پروتئین سرم در بیماران کاوازاکی قبل و بعد از درمان ایمونوگلوبولین
دانلود مقاله + سفارش ترجمه
دانلود مقاله ISI انگلیسی
رایگان برای ایرانیان
کلمات کلیدی
بیماری کاوازاکی، ایمونوگلوبولین داخل وریدی، پروتئوم پروتئین های مختلف بیان شده،
موضوعات مرتبط
علوم زیستی و بیوفناوری
بیوشیمی، ژنتیک و زیست شناسی مولکولی
زیست شیمی
چکیده انگلیسی
Kawasaki disease (KD) is a systemic vasculitis that mainly affects children younger than 5Â years. The causal pathogen is unknown, therefore specific diagnostic biomarkers and therapy are unavailable. High-dose intravenous immunoglobulin (IVIG) is considered as the most effective therapy to reduce the prevalence of coronary artery lesion (CAL) in KD; however, it has side effects. This study aimed to (1) determine whether IVIG therapy is effective at the molecular level; (2) provide the first serum proteomic profile of KD under IVIG therapy; and (3) screen for monitoring biomarker candidates. We extracted serum proteins from samples of healthy individuals and from KD patients before and after IVIG therapy, and employed two-dimensional electrophoresis and MALDI-TOF/TOF mass spectrometry to identify differentially expressed proteins. The identifications were validated by Western blotting. We identified 29 differentially expressed proteins in KD patients and found that IVIG therapy restored most of these proteins to near-normal levels. Tracing the protein levels of single patients before and after IVIG therapy showed that the proteins, especially Transthyretin (TTR), are potential markers for therapeutic monitoring. Functional analyses of these proteins by PANTHER and String suggested that the key influence of KD lay in the immune system, which was targeted by IVIG.
ناشر
Database: Elsevier - ScienceDirect (ساینس دایرکت)
Journal: Biochemical and Biophysical Research Communications - Volume 447, Issue 1, 25 April 2014, Pages 19-25
Journal: Biochemical and Biophysical Research Communications - Volume 447, Issue 1, 25 April 2014, Pages 19-25
نویسندگان
Li Zhang, Hong-Ling Jia, Wei-Min Huang, Chao-Wu Liu, Liang Hua, Te-Chang Liu, Li-Jia Mao, Yu-Fen Xu, Wei Li, Shu-Liang Xia, Ying-Yan Gan, Li Deng, Gong Zhang,