کد مقاله | کد نشریه | سال انتشار | مقاله انگلیسی | نسخه تمام متن |
---|---|---|---|---|
10755981 | 1050379 | 2014 | 6 صفحه PDF | دانلود رایگان |
عنوان انگلیسی مقاله ISI
Stable SREBP-1a knockdown decreases the cell proliferation rate in human preadipocyte cells without inducing senescence
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کلمات کلیدی
H3K9me3cyclin-dependent kinase inhibitor 2BHistone H3 lysine 9 trimethylationCDKN2BSGBSSREBP-1CDKN1ASREBPSA-β-gal3-(4,5-dimethyl-thiazol-2-yl)-2,5-diphenyltetrazolium bromideshRNAMTT - MTTshort hairpin RNA - RNA موی سر کوتاهsenescence-associated β-galactosidase - β-گالاکتوزیداز وابسته به پیریProliferation - ترویجcyclin-dependent kinase inhibitor 1A - مهار کننده 1A کیناز وابسته به کینازsterol regulatory element binding protein - پروتئین اتصال دهنده عصاره استرولSenescence - پیری
موضوعات مرتبط
علوم زیستی و بیوفناوری
بیوشیمی، ژنتیک و زیست شناسی مولکولی
زیست شیمی
پیش نمایش صفحه اول مقاله
چکیده انگلیسی
Sterol regulatory element binding proteins (SREBP), encoded by the Srebf1 and Srebf2 genes, are important regulators of genes involved in cholesterol and fatty acid metabolism. Whereas SREBP-2 controls the cholesterol synthesis, SREBP-1 proteins (-1a and -1c) function as the central hubs in lipid metabolism. Despite the key function of these transcription factors to promote adipocyte differentiation, the roles of SREBP-1 proteins during the preadipocyte state remain unknown. Here, we evaluate the role of SREBP-1 in preadipocyte proliferation using RNA interference technology. Knockdown of the SREBP-1a gene decreased the proliferation rate in human SGBS preadipocyte cell strain without inducing senescence. Furthermore, our data identified retinoblastoma binding protein 8 and cyclin-dependent kinase inhibitor 3 genes as new potential SREBP-1 targets, in addition to cyclin-dependent kinase inhibitor 1A which had already been described as a gene regulated by SREBP-1a. These data suggested a new role of SREBP-1 in adipogenesis via regulation of preadipocyte proliferation.
ناشر
Database: Elsevier - ScienceDirect (ساینس دایرکت)
Journal: Biochemical and Biophysical Research Communications - Volume 447, Issue 1, 25 April 2014, Pages 51-56
Journal: Biochemical and Biophysical Research Communications - Volume 447, Issue 1, 25 April 2014, Pages 51-56
نویسندگان
MarÃa Soledad Alvarez, Ana Fernandez-Alvarez, Carme Cucarella, Marta Casado,