کد مقاله | کد نشریه | سال انتشار | مقاله انگلیسی | نسخه تمام متن |
---|---|---|---|---|
10756061 | 1050381 | 2014 | 7 صفحه PDF | دانلود رایگان |
عنوان انگلیسی مقاله ISI
Lipid synthesis is promoted by hypoxic adipocyte-derived exosomes in 3T3-L1 cells
دانلود مقاله + سفارش ترجمه
دانلود مقاله ISI انگلیسی
رایگان برای ایرانیان
کلمات کلیدی
ACCFBSHSP72G6PDACNExosomeGAPDHHsc70heat shock cognate 71 kDa proteinHIF-1αHspAdipocytes - آدیپوسیتde novo lipogenesis - از لیپوژنز جدیدAcetonitrile - استونیتریلacetyl-CoA carboxylase - استیل کروکسی سیلازfatty acid synthase - اسید چرب سنتازProteomic analysis - تجزیه پروتئومیکسfetal bovine serum - سرم جنین گاوhypoxia-inducible factor-1α - عامل القاء شده با هیپوکسی 1αFasn - فسادwild-type - نوع وحشیHypoxia - هیپوکسیHeat shock protein - پروتئین شوک حرارتglucose-6-phosphate dehydrogenase - گلوکز 6-فسفات دهیدروژنازglyceraldehyde-phosphate dehydrogenase - گلیسرالیدید فسفات دهیدروژناز
موضوعات مرتبط
علوم زیستی و بیوفناوری
بیوشیمی، ژنتیک و زیست شناسی مولکولی
زیست شیمی
پیش نمایش صفحه اول مقاله
چکیده انگلیسی
Hypoxia occurs within adipose tissues as a result of adipocyte hypertrophy and is associated with adipocyte dysfunction in obesity. Here, we examined whether hypoxia affects the characteristics of adipocyte-derived exosomes. Exosomes are nanovesicles secreted from most cell types as an information carrier between donor and recipient cells, containing a variety of proteins as well as genetic materials. Cultured differentiated 3T3-L1 adipocytes were exposed to hypoxic conditions and the protein content of the exosomes produced from these cells was compared by quantitative proteomic analysis. A total of 231 proteins were identified in the adipocyte-derived exosomes. Some of these proteins showed altered expression levels under hypoxic conditions. These results were confirmed by immunoblot analysis. Especially, hypoxic adipocyte-released exosomes were enriched in enzymes related to de novo lipogenesis such as acetyl-CoA carboxylase, glucose-6-phosphate dehydrogenase, and fatty acid synthase (FASN). The total amount of proteins secreted from exosomes increased by 3-4-fold under hypoxic conditions. Moreover, hypoxia-derived exosomes promoted lipid accumulation in recipient 3T3-L1 adipocytes, compared with those produced under normoxic conditions. FASN levels were increased in undifferentiated 3T3-L1 cells treated with FASN-containing hypoxic adipocytes-derived exosomes. This is a study to characterize the proteomic profiles of adipocyte-derived exosomes. Exosomal proteins derived from hypoxic adipocytes may affect lipogenic activity in neighboring preadipocytes and adipocytes.
ناشر
Database: Elsevier - ScienceDirect (ساینس دایرکت)
Journal: Biochemical and Biophysical Research Communications - Volume 445, Issue 2, 7 March 2014, Pages 327-333
Journal: Biochemical and Biophysical Research Communications - Volume 445, Issue 2, 7 March 2014, Pages 327-333
نویسندگان
Soichi Sano, Yasukatsu Izumi, Takehiro Yamaguchi, Takanori Yamazaki, Masako Tanaka, Masayuki Shiota, Mayuko Osada-Oka, Yasuhiro Nakamura, Min Wei, Hideki Wanibuchi, Hiroshi Iwao, Minoru Yoshiyama,