کد مقاله | کد نشریه | سال انتشار | مقاله انگلیسی | نسخه تمام متن |
---|---|---|---|---|
10758309 | 1050406 | 2013 | 7 صفحه PDF | دانلود رایگان |
عنوان انگلیسی مقاله ISI
A mental retardation gene, motopsin/prss12, modulates cell morphology by interaction with seizure-related gene 6
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کلمات کلیدی
5-bromo-4-chloro-3-indolyl-α-d-galactopyranosideX-α-GalIPTGADENeurotrypsinSRCRPBS-TAdenine - آدنینisopropyl β-D-1-thiogalactopyranoside - ایزوپروپیل β-D-1-thiogalactopyranosideanalysis of variance - تحلیل واریانسANOVA - تحلیل واریانس Analysis of varianceCub - تولهshort consensus repeat - تکرار اجماع کوتاهDendrites - دندریت هاDIV - دیوdays in vitro - روز in vitropostnatal day - روز پس از زایمانSerine protease - سرین پروتئازphosphate-buffered saline containing 0.3% Triton X-100 - فسفات بافر حاوی 0.3٪ تریتون X-100 استScavenger receptor cysteine-rich - گیرنده گیرنده غنی از سستیونSCR - یکسوساز کنترلشده با سیلیکون
موضوعات مرتبط
علوم زیستی و بیوفناوری
بیوشیمی، ژنتیک و زیست شناسی مولکولی
زیست شیمی
پیش نمایش صفحه اول مقاله
چکیده انگلیسی
A serine protease, motopsin (prss12), plays a significant role in cognitive function and the development of the brain, since the loss of motopsin function causes severe mental retardation in humans and enhances social behavior in mice. Motopsin is activity-dependently secreted from neuronal cells, is captured around the synaptic cleft, and cleaves a proteoglycan, agrin. The multi-domain structure of motopsin, consisting of a signal peptide, a proline-rich domain, a kringle domain, three scavenger receptor cysteine-rich domains, and a protease domain at the C-terminal, suggests the interaction with other molecules through these domains. To identify a protein interacting with motopsin, we performed yeast two-hybrid screening and found that seizure-related gene 6 (sez-6), a transmembrane protein on the plasma membrane of neuronal cells, bound to the proline-rich/kringle domain of motopsin. Pull-down and immunoprecipitation analyses indicated the interaction between these proteins. Immunocytochemical and immunohistochemical analyses suggested the co-localization of motopsin and sez-6 at neuronal cells in the developmental mouse brain and at motor neurons in the anterior horn of human spinal cords. Transient expression of motopsin in neuro2a cells increased the number and length of neurites as well as the level of neurite branching. Interestingly, co-expression of sez-6 with motopsin restored the effect of motopsin at the basal level, while sez-6 expression alone showed no effects on cell morphology. Our results suggest that the interaction of motopsin and sez-6 modulates the neuronal cell morphology.
ناشر
Database: Elsevier - ScienceDirect (ساینس دایرکت)
Journal: Biochemical and Biophysical Research Communications - Volume 436, Issue 4, 12 July 2013, Pages 638-644
Journal: Biochemical and Biophysical Research Communications - Volume 436, Issue 4, 12 July 2013, Pages 638-644
نویسندگان
Shinichi Mitsui, Chiharu Hidaka, Mutsuo Furihata, Yoji Osako, Kazunari Yuri,