کد مقاله | کد نشریه | سال انتشار | مقاله انگلیسی | نسخه تمام متن |
---|---|---|---|---|
10758355 | 1050406 | 2013 | 8 صفحه PDF | دانلود رایگان |
عنوان انگلیسی مقاله ISI
Characterization of the oncogenic function of centromere protein F in hepatocellular carcinoma
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کلمات کلیدی
Mad1centromere protein FDABG2/M transitionSHC1TMAqRT-PCRsiRNAsBub1HCC - HCCsmall interfering RNAs - RNA های تداخل کوچکImmunohistochemistry - ایمونوهیستوشیمیIHC - ایمونوهیستوشیمیdiaminobenzidine - دیامینو بنزیدینPrognosis factor - عامل پیش آگهیMad2 - مد 2Tissue microarray - میکروآرشی بافتHematoxylin and Eosin - هماتوکسیلین و ائوزینquantitative real time polymerase chain reaction - واکنش زنجیره ای پلیمراز واقعی در زمان واقعیHepatocellular carcinoma - کارسینوم هپاتوسلولار(کارسینوم سلولهای استخوانی)
موضوعات مرتبط
علوم زیستی و بیوفناوری
بیوشیمی، ژنتیک و زیست شناسی مولکولی
زیست شیمی
پیش نمایش صفحه اول مقاله
چکیده انگلیسی
Centromere protein F (CENPF) is an essential nuclear protein associated with the centromere-kinetochore complex and plays a critical role in chromosome segregation during mitosis. Up-regulation of CENPF expression has previously been detected in several solid tumors. In this study, we aim to study the expression and functional role of CENPF in hepatocellular carcinoma (HCC). We found CENPF was frequently overexpressed in HCC as compared with non-tumor tissue. Up-regulated CENPF expression in HCC was positively correlated with serum AFP, venous invasion, advanced differentiation stage and a shorter overall survival. Cox regression analysis found that overexpression of CENPF was an independent prognosis factor in HCC. Functional studies found that silencing CENPF could decrease the ability of the cells to proliferate, form colonies and induce tumor formation in nude mice. Silencing CENPF also resulted in the cell cycle arrest at G2/M checkpoint by down-regulating cell cycle proteins cdc2 and cyclin B1. Our data suggest that CENPF is frequently overexpressed in HCC and plays a critical role in driving HCC tumorigenesis.
ناشر
Database: Elsevier - ScienceDirect (ساینس دایرکت)
Journal: Biochemical and Biophysical Research Communications - Volume 436, Issue 4, 12 July 2013, Pages 711-718
Journal: Biochemical and Biophysical Research Communications - Volume 436, Issue 4, 12 July 2013, Pages 711-718
نویسندگان
Yongdong Dai, Lulu Liu, Tingting Zeng, Ying-Hui Zhu, Jiangchao Li, Leilei Chen, Yan Li, Yun-Fei Yuan, Stephanie Ma, Xin-Yuan Guan,