کد مقاله | کد نشریه | سال انتشار | مقاله انگلیسی | نسخه تمام متن |
---|---|---|---|---|
10759380 | 1050422 | 2013 | 7 صفحه PDF | دانلود رایگان |
عنوان انگلیسی مقاله ISI
Cell diameter measurements obtained with a handheld cell counter could be used as a surrogate marker of G2/M arrest and apoptosis in colon cancer cell lines exposed to SN-38
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کلمات کلیدی
MSIWSTSN-385-bromo-2′-deoxyuridine - 5-bromo-2'-deoxyuridineG2/M arrest - G2 / M دستگیریMTT - MTTNAD(P)H - NAD (P) HBrdU - بروموداکسی اوریدینMicrosatellite instability - بی ثباتی ریزماهواره ایApoptosis - خزان یاختهایcolon cancer cell - سلول سرطانی کولونcoefficients of variation - ضرایب تنوعCell diameter - قطر سلولیnicotinamide adenine dinucleotide (phosphate) - نیکوتین آمید adenine dinucleotide (فسفات)Propidium iodide - پروتئین یدید
موضوعات مرتبط
علوم زیستی و بیوفناوری
بیوشیمی، ژنتیک و زیست شناسی مولکولی
زیست شیمی
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چکیده انگلیسی
In vitro assessment of chemosensitivity are important for experiments evaluating cancer therapies. The Scepter 2.0 cell counter, an automated handheld device based on the Coulter principle of impedance-based particle detection, enables the accurate discrimination of cell populations according to cell size and volume. In this study, the effects of SN-38, the active metabolite of irinotecan, on the colon cancer cell lines HCT116 and HT29 were evaluated using this device. The cell count data obtained with the Scepter counter were compared with those obtained with the 3H-thymidine uptake assay, which has been used to measure cell proliferation in many previous studies. In addition, we examined whether the changes in the size distributions of these cells reflected alterations in the frequency of cell cycle arrest and/or apoptosis induced by SN-38 treatment. In our experiments using the Scepter 2.0 cell counter, the cell counts were demonstrated to be accurate and reproducible measure and alterations of cell diameter reflected G2/M cell cycle arrest and apoptosis. Our data show that easy-to-use cell counting tools can be utilized to evaluate the cell-killing effects of novel treatments on cancer cells in vitro.
ناشر
Database: Elsevier - ScienceDirect (ساینس دایرکت)
Journal: Biochemical and Biophysical Research Communications - Volume 434, Issue 4, 17 May 2013, Pages 753-759
Journal: Biochemical and Biophysical Research Communications - Volume 434, Issue 4, 17 May 2013, Pages 753-759
نویسندگان
Makiko Tahara, Takeshi Inoue, Yasuyuki Miyakura, Hisanaga Horie, Yoshikazu Yasuda, Hirofumi Fujii, Kenjiro Kotake, Kokichi Sugano,