Generation and characterization of Tmeff2 mutant mice

► A Tmeff2 knock-in/null mouse line is generated (Tmeff2PLAP/PLAP). ► Tmeff2 mutant mice show growth retardation and die around weaning age. ► Tmeff2PLAP allele enables visualization of neuronal innervation with PLAP-staining. ► Tmeff2 mutant mice have normally developed central and peripheral nervous system. ► No in vivo evidence was found for a role of TMEFF2 as a tumor suppressor.