Label-free impedance responses of endogenous and synthetic chemokine receptor CXCR3 agonists correlate with Gi-protein pathway activation

► CXCL9 is a biased agonist on CXCR3. ► CXCR3 signaling can be detected using label-free impedance technology. ► CXCR3-induced impedance changes are mediated via Gαi-proteins. ► Impedance measurements reveal differences in signaling kinetics of various ligands. ► Impedance measurements reveal CXCR3-independent responses by CXCL9 and VUF10661.