Biochemical characterization of recombinant Candida albicans mannosyltransferases Mnt1, Mnt2 and Mnt5 reveals new functions in O- and N-mannan biosynthesis

► Mnt5 adds the second residues of lateral branches of the N-linked mannan outer chain. ► Mnt1 and Mnt2 participate in the addition of the 4° and 5° residues of O-linked mannan. ► Candida albicansO-linked mannans can be extended further than five mannose residues.