Transcription of the Tollip gene is elevated in intestinal epithelial cells through impaired O-GlcNAcylation-dependent nuclear translocation of the negative regulator Elf-1

► Transcriptional activation of the Tollitip gene is higher in IECs than in monocytes. ► Nt -194/-186 region acts as a cis-element and is recognized by Elf-1. ► Elf-1 suppresses Tollip gene transcription in monocytes but not in IECs. ► O-GlcNAc modification is necessary for nuclear translocation of Elf-1. ► O-GlcNAcylation-dependent nuclear translocation of Elf-1 is impaired in IECs.