Characterization of the AT180 epitope of phosphorylated Tau protein by a combined nuclear magnetic resonance and fluorescence spectroscopy approach

► pThr231 of the Tau protein is necessary for the binding of the AT180 antibody. ► pSer235 of the Tau protein does not interfere with the AT180 recognition of pThr231. ► Epitope mapping is efficiently achieved by combining NMR and FRET spectroscopy.