The NDUFB6 subunit of the mitochondrial respiratory chain complex I is required for electron transfer activity: A proof of principle study on stable and controlled RNA interference in human cell lines

► NDUFB6 is required for activity of mitochondrial complex I in human cell lines. ► Lentivirus based RNA interference results in frequent off target insertions. ► Flp-In recombinase mediated miRNA insertion allows gene-specific extinction.