Accelerated cellular senescence phenotype of GAPDH-depleted human lung carcinoma cells

► We examined the effect of glyceraldehyde 3-phosphate (GAPDH) depletion on proliferation of human carcinoma A549 cells. ► GAPDH depletion induces accelerated senescence in tumor cells via AMPK network, in the absence of DNA damage. ► Metabolic and genetic rescue experiments indicate that GAPDH has regulatory functions linking energy metabolism and cell cycle. ► Induction of senescence in LKB1-deficient lung cancer cells via GAPDH depletion suggests a novel strategy to control tumor cell proliferation.