Cloning and heterologous expression of new xANO2 from Xenopus laevis

► Novel different anoctamin (TMEM16) of calcium-activated chloride channel (CaCC) in Xenopus laevis. ► xANO2 has the distinct characteristics in primary structure and expression pattern. ► Ubiquitous expression profiles in Xenopus tissues. ► xANO2 shows the clear channel activity and sensitive inhibition by NFA compared to xTMEM16A.