Overcoming the heterologous bias: An in vivo functional analysis of multidrug efflux transporter, CgCdr1p in matched pair clinical isolates of Candida glabrata

► First report to demonstrate an in vivo expression system of an ABC multidrug transporter CgCdr1p of C. glabrata. ► First report on the structure and functional characterization of CgCdr1p. ► Functional conservation of divergent but typical residues of CgCdr1p. ► CgCdr1p elicits promiscuity towards substrates and has a large drug binding pocket with overlapping specificities.