Characterization and structure identification of an antimicrobial peptide, hominicin, produced by Staphylococcus hominis MBBL 2-9

► Antimicrobial peptide, hominicin, was purified from culture supernatant of Staphylococcus hominis MBBL 2-9. ► Hominicin exhibited potent activity against several Gram-positive and antibiotic-resistant pathogens. ► Hominicin exhibited heat stability up to 121 °C for 15 min and activity under both acidic and basic conditions (from pH 2.0 to 10.0). ► The sequence of hominicin was revealed as DmIle-Dhb-Pro-Ala-Dhb-Pro-Phe-Dhb-Pro-Ala-Ile-Thr-Glu-Ile-Dhb-Ala-Ala-Val-Ile-Ala-Dmp. ► Hominicin is a novel antimicrobial peptide, which has uncommon amino acid residues like the ones in Class I group, but without thioether bridge.