Redox-controlled backbone dynamics of human cytochrome c revealed by 15N NMR relaxation measurements

► The dynamic parameters for the backbone dynamics in Cyt c were determined. ► The backbone mobility of Cyt c is highly restricted due to the covalently bound heme. ► The backbone mobility of Cyt c is more restricted upon the oxidation of the heme. ► The redox-dependent dynamics are shown in the backbone of Cyt c. ► The backbone dynamics of Cyt c would regulate the electron transfer from Cyt c.