کد مقاله کد نشریه سال انتشار مقاله انگلیسی نسخه تمام متن
10765399 1050592 2010 5 صفحه PDF دانلود رایگان
عنوان انگلیسی مقاله ISI
5′-AZA induces Ngn3 expression and endocrine differentiation in the PANC-1 human ductal cell line
موضوعات مرتبط
علوم زیستی و بیوفناوری بیوشیمی، ژنتیک و زیست شناسی مولکولی زیست شیمی
پیش نمایش صفحه اول مقاله
5′-AZA induces Ngn3 expression and endocrine differentiation in the PANC-1 human ductal cell line
چکیده انگلیسی
Neurogenin 3 is necessary for endocrine cell development in the embryonic pancreas and has been shown to induce transdifferentiation duct cells from adult pancreas toward a neuro-endocrine phenotype. Here we discovered that the demethylating agent 5′-Azadeoxycytidine (AZA) induced Ngn3 expression and endocrine differentiation from the PANC-1 human ductal cell line. The expression of markers specific to mature islet cells, i.e., glucagon and somatostatin, was also observed. In addition, we demonstrated that growth factors (betacellulin and soluble factors released during pancreas embryogenesis) increased the level of maturation. Our studies revealed that the PANC-1 model system may provide a basis for elucidating the ductal/endocrine differentiation.
ناشر
Database: Elsevier - ScienceDirect (ساینس دایرکت)
Journal: Biochemical and Biophysical Research Communications - Volume 391, Issue 1, 1 January 2010, Pages 305-309
نویسندگان
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