کد مقاله | کد نشریه | سال انتشار | مقاله انگلیسی | نسخه تمام متن |
---|---|---|---|---|
10765669 | 1050594 | 2009 | 4 صفحه PDF | دانلود رایگان |
عنوان انگلیسی مقاله ISI
Effective isolation of RNA aptamer through suppression of PCR bias
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کلمات کلیدی
HEPESSELEX4-(2-hydroxyethyl)-1-piperazineethanesulfonic acid - 4- (2-hydroxyethyl) -1-piperazineethanesulfonic acidAptamer - آپتامرهاRNA - اسید ریبونوکلئیکAmplification - تقویتsystematic evolution of ligands by exponential enrichment - تکامل سیستماتیک لیگاند ها با غنی سازی نمایشیTranscription - رونویسیBias - سوگیری یا سوگراییPCR - واکنش زنجیرهٔ پلیمراز
موضوعات مرتبط
علوم زیستی و بیوفناوری
بیوشیمی، ژنتیک و زیست شناسی مولکولی
زیست شیمی
پیش نمایش صفحه اول مقاله

چکیده انگلیسی
An aptamer is a short RNA or DNA molecule that binds to a specific target. The main strategy for obtaining aptamers is systematic evolution of ligands by exponential enrichment (SELEX). Although various SELEX techniques have been devised and refined on the basis of the selection technique used, in most cases, the isolation of an aptamer still requires several trials or the use of special equipment. In the present study, we attempted SELEX in which PCR bias was suppressed by using RNA transcription to amplify nucleic acids. This procedure, which can be accomplished easily and inexpensively without special equipment, effectively simplifies the SELEX process. Using this SELEX, we obtained large numbers of RNA aptamers against the target that could not be isolated by standard SELEX. The results of our study suggest that exclusion of PCR bias may be far more important than previously assumed for isolating RNA aptamers via SELEX.
ناشر
Database: Elsevier - ScienceDirect (ساینس دایرکت)
Journal: Biochemical and Biophysical Research Communications - Volume 386, Issue 1, 14 August 2009, Pages 223-226
Journal: Biochemical and Biophysical Research Communications - Volume 386, Issue 1, 14 August 2009, Pages 223-226
نویسندگان
Shoutaro Tsuji, Naomi Hirabayashi, Shintaro Kato, Joe Akitomi, Hazuki Egashira, Taku Tanaka, Iwao Waga, Takashi Ohtsu,