کد مقاله | کد نشریه | سال انتشار | مقاله انگلیسی | نسخه تمام متن |
---|---|---|---|---|
10766618 | 1050655 | 2008 | 6 صفحه PDF | دانلود رایگان |
عنوان انگلیسی مقاله ISI
Different inhibitory response of cyanidin and methylene blue for filament formation of tau microtubule-binding domain
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کلمات کلیدی
Microtubule-binding domainPHFNFTMBDTHs - THSInhibition - بازداریAlzheimer’s disease - بیماری آلزایمرThioflavin S - تیوفلاوین SHIS - خودFilament - رشتهPaired helical filament - رشته مارپیچ دوار شدهcircular dichroism - رنگ تابی دورانیMicrotubule - ریزلوله یا میکروتوبولCyanidin - سایانیدینMethylene blue - متیلن آبیElectron microscopy - میکروسکوپ الکترونیmap - نقشهhistidine - هیستیدینmicrotubule-associated protein - پروتئین مرتبط با میکروتوبول
موضوعات مرتبط
علوم زیستی و بیوفناوری
بیوشیمی، ژنتیک و زیست شناسی مولکولی
زیست شیمی
پیش نمایش صفحه اول مقاله
چکیده انگلیسی
One of the priorities in Alzheimer research is to develop a compound that inhibits the filament formation of tau protein. Since the three- or four-repeat microtubule-binding domain (MBD) in tau protein plays an essential role in filament formation, the inhibitory behavior of cyanidin (Cy) and methylene blue (MB) with respect to heparin-induced filament formation of MBD in a neutral solution (pH 7.6) was characterized by fluorescence, circular dichroism, and electron microscopy measurements. The planar aromatic ring of Cy and the N-unsubstituted phenothiazine ring of MB were shown to be necessary for the inhibition. However, the inhibitory responses with respect to heparin-induced filament formation to the second and third repeat peptides of MBD were different: Cy suppresses the formation and MB does not prevent the formation. This suggests the importance of the first and fourth repeat peptides in the inhibitory activity of MB for MBD filament formation. In this study, we showed that the decrease of thioflavin S fluorescence intensity is not always linked to inhibition of filament formation.
ناشر
Database: Elsevier - ScienceDirect (ساینس دایرکت)
Journal: Biochemical and Biophysical Research Communications - Volume 374, Issue 1, 12 September 2008, Pages 158-163
Journal: Biochemical and Biophysical Research Communications - Volume 374, Issue 1, 12 September 2008, Pages 158-163
نویسندگان
Masaki Hattori, Etsuko Sugino, Katsuhiko Minoura, Yasuko In, Miho Sumida, Taizo Taniguchi, Koji Tomoo, Toshimasa Ishida,