کد مقاله | کد نشریه | سال انتشار | مقاله انگلیسی | نسخه تمام متن |
---|---|---|---|---|
10767026 | 1050696 | 2007 | 4 صفحه PDF | دانلود رایگان |
عنوان انگلیسی مقاله ISI
13C-enrichment at carbons 8 and 2 of uric acid after 13C-labeled folate dose in man
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کلمات کلیدی
موضوعات مرتبط
علوم زیستی و بیوفناوری
بیوشیمی، ژنتیک و زیست شناسی مولکولی
زیست شیمی
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چکیده انگلیسی
To evaluate folate-dependent carbon incorporation into the purine ring, we measured 13C-enrichment independently at C2 and C8 of urinary uric acid (the final catabolite of purines) in a healthy male after an independent oral dose of [6RS]-5-[13C]-formyltetrahydrofolate ([6RS]-5-H13CO-H4folate) or 10-H13CO-7,8-dihydrofolate (10-H13CO-H2folate). The C2 position was 13C-enriched more than C8 after [6RS]-5-H13CO-H4folate, and C2 was exclusively enriched after 10-H13CO-H2folate. The enrichment of C2 was greater from [6RS]-5-H13CO-H4folate than 10-H13CO-H2folate using equimolar bioactive doses. Our data suggest that formyl C of [6RS]-10-H13CO-H4folate was not equally utilized by glycinamide ribotide transformylase (enriches C8) and aminoimidazolecarboxamide ribotide (AICAR) transformylase (enriches C2), and the formyl C of 10-H13CO-H2folate was exclusively used by AICAR transformylase. 10-HCO-H2folate may function in vivo as the predominant substrate for AICAR transformylase in humans.
ناشر
Database: Elsevier - ScienceDirect (ساینس دایرکت)
Journal: Biochemical and Biophysical Research Communications - Volume 361, Issue 2, 21 September 2007, Pages 307-310
Journal: Biochemical and Biophysical Research Communications - Volume 361, Issue 2, 21 September 2007, Pages 307-310
نویسندگان
Joseph E. Baggott, Gregory S. Gorman, Sarah L. Morgan, Tsunenobu Tamura,