کد مقاله | کد نشریه | سال انتشار | مقاله انگلیسی | نسخه تمام متن |
---|---|---|---|---|
10767290 | 1050718 | 2007 | 6 صفحه PDF | دانلود رایگان |
عنوان انگلیسی مقاله ISI
Effect of KAI1/CD82 on the β1 integrin maturation in highly migratory carcinoma cells
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کلمات کلیدی
ECMKAI1/CD82H1299Duffy Antigen Receptor for ChemokinesTGF-βSfMFAKTM4SFmAbPBSBSA - BSAHGF/SF - HGF / SFbovine serum albumin - آلبومین سرم گاوMonoclonal antibody - آنتی بادی مونوکلونالIntegrin β1 - انتگرین β1transforming growth factor-β - تبدیل فاکتور رشد βDARC - دارسیSerum free media - رسانه آزاد سرمTumor suppressor - سرکوبگر تومورendoplasmic reticulum - شبکه آندوپلاسمی Hepatocyte growth factor/scatter factor - عامل رشد هپاتوسیت / عامل پراکندگیTransmembrane 4 superfamily - فراملی 4 خانواده فوق العادهFibronectin - فیبرونکتینLaminin - لامینینExtracellular matrix - ماتریکس خارج سلولیPhosphate-buffered saline - محلول نمک فسفات با خاصیت بافریCell migration - مهاجرت سلولیfocal adhesion kinase - کیناز چسبندگی کانونی
موضوعات مرتبط
علوم زیستی و بیوفناوری
بیوشیمی، ژنتیک و زیست شناسی مولکولی
زیست شیمی
پیش نمایش صفحه اول مقاله

چکیده انگلیسی
The KAI1/CD82 protein has been documented as the tumor metastasis suppressor in many types of human cancers. KAI1/CD82 regulates cell motility and invasiveness; however, the mechanism by which this occurs remains to be fully established. Several studies have shown that KAI1/CD82 modulates integrin-dependent signaling. It was suggested that KAI1/CD82 might function to attenuate the β1 integrin function of inducing cellular migration. A wound-healing and modified Boyden chamber assays were performed to investigate the mechanism of the KAI1/CD82-mediated inhibition of cell migration. It was found that the migratory ability of H1299/CD82 was inhibited. The immunoblotting and biotinylation assays revealed that H1299/CD82 showed significantly decreased expression of the mature form of β1, which was functional at the cell surface. It was confirmed that KAI1/CD82 regulates the maturation of the β1 integrin using CD82-specific si-RNA. These results support a model in which KAI1/CD82 attenuates the maturation of the β1 integrin precursor and thereby suppresses cell migration.
ناشر
Database: Elsevier - ScienceDirect (ساینس دایرکت)
Journal: Biochemical and Biophysical Research Communications - Volume 359, Issue 3, 3 August 2007, Pages 703-708
Journal: Biochemical and Biophysical Research Communications - Volume 359, Issue 3, 3 August 2007, Pages 703-708
نویسندگان
Bo Keun Jee, Joo Yong Lee, Young Lim, Kweon Haeng Lee, Yang-Hyeok Jo,