کد مقاله | کد نشریه | سال انتشار | مقاله انگلیسی | نسخه تمام متن |
---|---|---|---|---|
10767837 | 1050799 | 2005 | 7 صفحه PDF | دانلود رایگان |
عنوان انگلیسی مقاله ISI
Minocycline up-regulates BCL-2 levels in mitochondria and attenuates male germ cell apoptosis
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کلمات کلیدی
موضوعات مرتبط
علوم زیستی و بیوفناوری
بیوشیمی، ژنتیک و زیست شناسی مولکولی
زیست شیمی
پیش نمایش صفحه اول مقاله
![عکس صفحه اول مقاله: Minocycline up-regulates BCL-2 levels in mitochondria and attenuates male germ cell apoptosis Minocycline up-regulates BCL-2 levels in mitochondria and attenuates male germ cell apoptosis](/preview/png/10767837.png)
چکیده انگلیسی
In this study, we determined the efficacy of minocycline, a second generation tetracycline, in preventing male germ cell apoptosis after withdrawal of gonadotropins and intratesticular testosterone (T). Groups of 5 male rats received one of the following treatments daily for 5 days: (i) daily sc injection of GnRH-A (1.6Â mg/kg BW), (ii) oral administration of 30% gum acacia as a vehicle control, and (iii) GnRH-AÂ +Â oral administration of 50 or 100Â mg/kg BW of minocycline. Minocycline at both 50 and 100Â mg dose levels significantly (PÂ <Â 0.05) prevented GnRH-A -induced germ cell apoptosis by 59.4% and 62.2%, respectively, and fully prevented PARP cleavage. Minocycline-mediated protection occurred at the mitochondria, involving the restoration of the BCL-2 levels and, in turn, suppression of cytochrome c and DIABLO release. Minocycline was also effective in preventing human male germ cell apoptosis induced by hormone free culture condition.
ناشر
Database: Elsevier - ScienceDirect (ساینس دایرکت)
Journal: Biochemical and Biophysical Research Communications - Volume 337, Issue 2, 18 November 2005, Pages 663-669
Journal: Biochemical and Biophysical Research Communications - Volume 337, Issue 2, 18 November 2005, Pages 663-669
نویسندگان
Mark Castanares, Yanira Vera, Krista Erkkilä, Sauli Kyttänen, Yanhe Lue, Leo Dunkel, Christina Wang, Ronald S. Swerdloff, Amiya P. Sinha Hikim,