کد مقاله | کد نشریه | سال انتشار | مقاله انگلیسی | نسخه تمام متن |
---|---|---|---|---|
10768760 | 1050814 | 2005 | 7 صفحه PDF | دانلود رایگان |
عنوان انگلیسی مقاله ISI
Functional cytoplasmic domains of the Mac-1 integrin receptor in phorbol ester-treated U937 cells
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کلمات کلیدی
موضوعات مرتبط
علوم زیستی و بیوفناوری
بیوشیمی، ژنتیک و زیست شناسی مولکولی
زیست شیمی
پیش نمایش صفحه اول مقاله
![عکس صفحه اول مقاله: Functional cytoplasmic domains of the Mac-1 integrin receptor in phorbol ester-treated U937 cells Functional cytoplasmic domains of the Mac-1 integrin receptor in phorbol ester-treated U937 cells](/preview/png/10768760.png)
چکیده انگلیسی
The integrin receptor Mac-1 regulates adherence and survival of activated tissue macrophages but the underlying molecular mechanisms are poorly understood. Phorbol ester-induced macrophagic differentiation in U937 cells leads to surface expression of Mac-1 and its activation as well. We have attempted to determine essential amino acids for these activities in the cytoplasmic regions of CD11b and CD18 subunits by deletion mutagenesis. There was complete correlation between adherence and survival. Those deletions that lead to loss of adherence and enhanced apoptosis are truncation of CD11b before the MSEGG sequence; CD18 internal deletion of either the membrane-proximal residues before the NPLF sequence or the NPLF sequence itself; CD18 truncation of the C-terminal residues after the NPLF sequence. Unexpectedly, when the NPLF sequence and the C-terminal residues were removed together by truncation, the adherent, antiapoptotic properties were restored. These results were discussed in terms of protein interaction with Mac-1 cytoplasmic regions.
ناشر
Database: Elsevier - ScienceDirect (ساینس دایرکت)
Journal: Biochemical and Biophysical Research Communications - Volume 335, Issue 3, 30 September 2005, Pages 858-864
Journal: Biochemical and Biophysical Research Communications - Volume 335, Issue 3, 30 September 2005, Pages 858-864
نویسندگان
Koichi Hamada, Hiroyasu Utiyama,