کد مقاله کد نشریه سال انتشار مقاله انگلیسی نسخه تمام متن
10769506 1050822 2005 9 صفحه PDF دانلود رایگان
عنوان انگلیسی مقاله ISI
Protection from oxidative stress by methionine sulfoxide reductases in RPE cells
موضوعات مرتبط
علوم زیستی و بیوفناوری بیوشیمی، ژنتیک و زیست شناسی مولکولی زیست شیمی
پیش نمایش صفحه اول مقاله
Protection from oxidative stress by methionine sulfoxide reductases in RPE cells
چکیده انگلیسی
We investigated the role of methionine sulfoxide reductases (Msrs) in oxidant-stress-induced cell death in retinal pigmented epithelial (RPE) cells. In RPE cells exposed to varying doses of H2O2, gene expression of MsrA and hCBS-1 (the human analog of MsrB2) increased in a dose-dependent and time-dependent manner with maximal increase with 150 μM H2O2 in 24 h. H2O2 treatment resulted in the generation of reactive oxygen species and activation of caspase 3. Confocal microscopic and protein analysis showed an increase in MsrA expression in cytosol and mitochondria. Silencing of MsrA resulted in caspase 3 induction and accentuated cell death from H2O2. Focal, strong immunoreactivity for MsrA was observed in sub-RPE macular drusen from patients with age-related macular degeneration. In summary, our data show that MsrA and hCBS-1 are up-regulated in oxidative stress to counteract injury to RPE.
ناشر
Database: Elsevier - ScienceDirect (ساینس دایرکت)
Journal: Biochemical and Biophysical Research Communications - Volume 334, Issue 1, 19 August 2005, Pages 245-253
نویسندگان
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