کد مقاله | کد نشریه | سال انتشار | مقاله انگلیسی | نسخه تمام متن |
---|---|---|---|---|
10769899 | 1050826 | 2005 | 7 صفحه PDF | دانلود رایگان |
عنوان انگلیسی مقاله ISI
Posttranslational processing of SREBP-1 in rat hepatocytes is regulated by insulin and cAMP
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کلمات کلیدی
موضوعات مرتبط
علوم زیستی و بیوفناوری
بیوشیمی، ژنتیک و زیست شناسی مولکولی
زیست شیمی
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![عکس صفحه اول مقاله: Posttranslational processing of SREBP-1 in rat hepatocytes is regulated by insulin and cAMP Posttranslational processing of SREBP-1 in rat hepatocytes is regulated by insulin and cAMP](/preview/png/10769899.png)
چکیده انگلیسی
Insulin and cAMP have opposing effects on de novo fatty acid synthesis in liver and in cultured hepatocytes mediated by sterol-regulatory element binding protein (SREBP). To determine whether these agents regulate the cleavage of full-length SREBP to generate the transcriptionally active N-terminal fragment (nSREBP) in primary rat hepatocytes, an adenoviral vector (Ad-SREBP-1a) was constructed to constitutively express full-length SREBP-1a. Insulin increased, and dibutyryl (db)-cAMP inhibited, generation of nSREBP-1a from its full-length precursor. Insulin stimulated processing of SREBP-1a within 1Â h, and the effect was sustained for at least 24Â h. The initial stimulation of SREBP processing by insulin preceded measurable reduction in Insig-2 mRNA levels. Rat hepatocytes were also infected with an adenovirus expressing the nuclear form of SREBP-1c (Ad-nSREBP-1c). Insulin increased the half-life of constitutively expressed nSREBP-1c, and this effect of insulin was also inhibited by db-cAMP.
ناشر
Database: Elsevier - ScienceDirect (ساینس دایرکت)
Journal: Biochemical and Biophysical Research Communications - Volume 332, Issue 1, 24 June 2005, Pages 174-180
Journal: Biochemical and Biophysical Research Communications - Volume 332, Issue 1, 24 June 2005, Pages 174-180
نویسندگان
Chandrahasa R. Yellaturu, Xiong Deng, Lauren M. Cagen, Henry G. Wilcox, Edwards A. Park, Rajendra Raghow, Marshall B. Elam,