کد مقاله | کد نشریه | سال انتشار | مقاله انگلیسی | نسخه تمام متن |
---|---|---|---|---|
10772053 | 1050847 | 2005 | 8 صفحه PDF | دانلود رایگان |
عنوان انگلیسی مقاله ISI
Pivotal participation of nitrogen dioxide in l-arginine induced acute necrotizing pancreatitis: protective role of superoxide scavenger 4-OH-TEMPO
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کلمات کلیدی
موضوعات مرتبط
علوم زیستی و بیوفناوری
بیوشیمی، ژنتیک و زیست شناسی مولکولی
زیست شیمی
پیش نمایش صفحه اول مقاله
چکیده انگلیسی
For the first time, a direct sensitive method of NO2 detection and measurement in biological material has been established. It is based on the interaction of this radical with the coordination compound of Cr(III) with aminodeoxysugar as biosensor. Our new method makes it possible to precisely assess NO2 level in experimental acute necrotizing pancreatitis induced by l-arginine, where oxidative and nitrosative stresses are supposed to play a key role in the pathomechanism of the disease. As much as 20Â nmol of NO2/mg protein was detected which correlated with severe deterioration of pancreatic acinar cell ultrastructure. Protective effect of superoxide radical scavenger 4-OH-TEMPO expressed as NO2 level decrease confirmed by preserved acinar cell ultrastructure and decreased pancreatic amylase release to blood serum is demonstrated. This study reveals a possible pathomechanism of l-arginine induced acute pancreatitis.
ناشر
Database: Elsevier - ScienceDirect (ساینس دایرکت)
Journal: Biochemical and Biophysical Research Communications - Volume 326, Issue 2, 14 January 2005, Pages 313-320
Journal: Biochemical and Biophysical Research Communications - Volume 326, Issue 2, 14 January 2005, Pages 313-320
نویسندگان
Aleksandra DÄ
browska, Dagmara Jacewicz, Agnieszka ÅapiÅska, Bogdan Banecki, Adam Figarski, MichaÅ SzkatuÅa, JÄdrzej Lehman, Jacek Krajewski, Jolanta Kubasik-Juraniec, MichaÅ Woźniak, Lech ChmurzyÅski,