کد مقاله | کد نشریه | سال انتشار | مقاله انگلیسی | نسخه تمام متن |
---|---|---|---|---|
10795405 | 1052579 | 2015 | 14 صفحه PDF | دانلود رایگان |
عنوان انگلیسی مقاله ISI
Direct energy transfer from the major antenna to the photosystem II core complexes in the absence of minor antennae in liposomes
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کلمات کلیدی
SQDGn-octyl-β-d-glucopyranosideDCMULHCIINPQβ-DDMOxygen evolution complexDPCChlOECTLCMGDGDGDGDCPIPMCC1,5-Diphenylcarbazide - 1،5-دیفینیل کربازید2,6-dichlorophenol indophenol - 2،6-dichlorophenol indophenol3-(3,4-Dichlorophenyl)-1,1-dimethylurea - 3- (3،4-Dichlorophenyl) -1،1-dimethylureadodecyl-β-D-maltoside - dodecyl-β-D-مالتوزیدProtein–protein interaction - تعامل پروتئین-پروتئینNon-photochemical quenching - خنک سازی غیر فتوشیمیاییdigalactosyl diacylglycerol - دیگالاکتوزیل دیسیل گلیسرولChlorophyll - سبزینه یا کلروفیلSulfoquinovosyl diacylglycerol - سولفووینوواسییل دیسیل گلیسرولPhotosystem - سیستم عکسPhotosystem II - فتوسیستم 2phosphatidylglycerol - فسفاتیدیل گلیسرولLight-harvesting complex - مجتمع جمع آوری نورreaction center - مرکز واکنشMonogalactosyl diacylglycerol - مونوگلاکتوزیل دیسیlglycerolProteoliposomes - پروتئولپوزوم هاProteoliposome - پروتئولیتوزومthin-layer chromatography - کروماتوگرافی نازک لایه
موضوعات مرتبط
علوم زیستی و بیوفناوری
علوم کشاورزی و بیولوژیک
دانش گیاه شناسی
پیش نمایش صفحه اول مقاله
چکیده انگلیسی
Minor antennae of photosystem (PS) II, located between the PSII core complex and the major antenna (LHCII), are important components for the structural and functional integrity of PSII supercomplexes. In order to study the functional significance of minor antennae in the energetic coupling between LHCII and the PSII core, characteristics of PSII-LHCII proteoliposomes, with or without minor antennae, were investigated. Two types of PSII preparations containing different antenna compositions were isolated from pea: 1) the PSII preparation composed of the PSII core complex, all of the minor antennae, and a small amount of major antennae (MCC); and 2) the purified PSII dimeric core complexes without periphery antenna (CC). They were incorporated, together with LHCII, into liposomes composed of thylakoid membrane lipids. The spectroscopic and functional characteristics were measured. 77Â K fluorescence emission spectra revealed an increased spectral weight of fluorescence from PSII reaction center in the CC-LHCII proteoliposomes, implying energetic coupling between LHCII and CC in the proteoliposomes lacking minor antennae. This result was further confirmed by chlorophyll a fluorescence induction kinetics. The incorporation of LHCII together with CC markedly increased the antenna cross-section of the PSII core complex. The 2,6-dichlorophenolindophenol photoreduction measurement implied that the lack of minor antennae in PSII supercomplexes did not block the energy transfer from LHCII to the PSII core complex. In conclusion, it is possible, in liposomes, that LHCII transfer energy directly to the PSII core complex, in the absence of minor antennae.
ناشر
Database: Elsevier - ScienceDirect (ساینس دایرکت)
Journal: Biochimica et Biophysica Acta (BBA) - Bioenergetics - Volume 1847, Issue 2, February 2015, Pages 248-261
Journal: Biochimica et Biophysica Acta (BBA) - Bioenergetics - Volume 1847, Issue 2, February 2015, Pages 248-261
نویسندگان
Ruixue Sun, Kun Liu, Lianqing Dong, Yuling Wu, Harald Paulsen, Chunhong Yang,